黑曲霉CICIM F0410中α-葡萄糖苷酶的酶学性质研究

Studies on Enzymatic Properties of Alpha-glucosidase in Aspergillus niger CICIM F0410

研究了黑曲霉(Aspergillus niger)CICIMF0410发酵粗酶液中α-葡萄糖苷酶的酶学性质,发现该酶最适反应温度为55℃,最适反应pH值5.0,在65℃以下保持酶活力相对稳定,且能在pH值(4.0～6.5)范围内保持相对较高的酶活力。以该菌株的染色体DNA为模板,PCR扩增出大小为3.1kb的片段,经序列比对发现与NCBI上已公布的黑曲霉(Aspergillus niger)CBS513.88(登录号XP_001402053)序列有2个碱基的区别:2272(A→G),3098(T→G),导致2个氨基酸残基的变化:687(Asn→Ser)、983(Ser→Arg)。

This paper reported the enzymatic properties of an α-glucosidase in the coarse of Aspergills niger CICIM F0410.The results showed that the enzyme had an optimum activity at 55℃ and pH 5.0. It was active below 65℃ after being incubated at pH 5.0 for 30 min and relatively stable at pHs ranging from 4.0 to 6.5. A bout 3.1 kb gene was amplified by the method of PCR which took the genomic DNA of CICIM F0410 as template. After being blasted at internet,it was founded that there were two different base pairs between CICIM F0410 and CBS 513.88（gene ID XP_001402053 ） ： 2272 （A→G）, 3098（T→G ）which leading to the change of two different anmio acids 687 （Asn→Ser）, 983（Ser→Arg）.