^(18)O内标质谱法比较定量蛋白质组学计量的数学算法

Mathematical Algorithms for Differential Quantitative Proteomics by Mass Spectrometry Using ^(18)O-labeled Internal Standards

18O内标蛋白质质谱定量分析是一种极具前景的比较蛋白组学研究技术,虽然该方法与迄今所有内标法相比有一系列优点,但是由于其质谱是由16O未标记品种,单及双18O标记品种分子同位素峰簇相互迭加所构成的复杂谱,定量分析的关键是发展有效的质谱解析算法。有鉴于此,综述和讨论了18O内标质谱差异比较蛋白质分析过程中,分子同位素簇分布的简化计算方法,以及质谱混和谱解析的数学方法。

Relative quantization of proteins by mass spectrometry using ^18O-labeled internal standards is a promising method for differential quantitative proteomics,having a series of advantages over other stable isotope labeling. But isotope clusters of ^18O-labeled species is overlaps with natural isotope clusters of non-labeled species,thus,effective algorithm is to be critical to quantitative analysis. Therefore,the mathematical method for analyzing ^18O-labeled peptides was summarized and discussed.