普罗帕酮的电还原行为及其应用

Electroreduction Behavior of Propafenone and Its Application

采用线性扫描极谱法研究了抗心率失常药物普罗帕酮在汞电极上的电还原行为,建立了测定实际样品中普罗帕酮的新方法。在pH<11.6底液中,普罗帕酮产生1个还原波;在底液pH≥11.6条件下,普罗帕酮产生P1和P22个还原波。研究表明,P1波为普罗帕酮产生的催化氢波,P2波为普罗帕酮分子中羰基的电化学还原波。在pH=12.3的Britton-Robinson缓冲溶液中,二阶导数极谱图上P2波的峰电流与普罗帕酮的浓度在9.6×10-8～4.0×10-6mol/L范围内呈线性关系,检出限为5×10-8mol/L。对1.0×10-6mol/L普罗帕酮测定6次的RSD=1.2%。用二阶导数极谱法测定片剂中普罗帕酮的含量结果与药典方法测得结果一致,血清中不同浓度普罗帕酮测定的回收率在98.6%～102%。

The electroreduction behavior of antiarrhythmic drug propafenone at Hg electrode was studied by means of linear sweep polarography, and a new determination method of propafenone in factual samples was developed. In solution with pH 〈 11.6, only one reduction peak was observed in the linear sweep polarogram and the mechanism of the peak was studied. In the solution with pH 〉 11.6, two reduction waves （ P1, P2 ） can be observed in the polarogram. P1 was proved to be a catalytic hydrogen wave and P2 was attributed to the reduction of propafenone itself. In a Britton-Robinson buffer （ pH = 12. 3 ）, a linear relationship can be established between the second order polarographic wave current of P1 and the concentration of propafenone in a range of 9.6 × 10^-8 to 4.0 × 10^-6 mol/L with a detection limit of 5 × 10^-8 mol/L. The RSD of 6 repeated measurements of 1.0 × 10^-6 mol/L propafenone was 1. 2%. The second order polarography was utilized to determine the content of propafenone in tablets and spiked serum. The determination results of propafenone in tablets by the second order derivative polarography were in agreement with the results obtained by UV spectro-photometry, and the recovery of propafenone in spiked serum was between in 98.6% to102%.