我国不同人群及各型肝炎病人庚型肝炎感染检测分析

HGV Infection in Different Populations and Patients with Various Liver Diseases of China

应用酶联免疫试验（ＥＩＡ）检测抗ＨＧＶ、ＨＢｓＡｇ、抗ＨＢｓ、抗ＨＢｃ和抗ＨＣＶ，对抗ＨＧＶ阳性的部分标本进一步用逆转录套式聚合酶链反应法（ＲＴｎＰＣＲ）检测ＨＧＶＲＮＡ。结果各类人群及肝炎病人抗ＨＧＶ阳性率分别是：一般人群１０％（４／３９５），献全血员３６％（１０／２７８），某单采浆点人群１２９％（７０／５４１），静脉毒瘾者１３５％（２２／１６２），血透析病人２１４％（１２／５６），ＨＢｓＡｇ携带者１２０％（３／２５），慢性乙型肝炎病人２６２％（１１／４０），慢性丙型肝炎病人１２５％（１７／１３６），肝硬化病人３６４％（４／１１），慢性非甲戊型肝炎病人１８８％（３／１６）。提示献血员、静脉毒瘾者、血透析病人及ＨＢＶ或ＨＣＶ感染者是ＨＧＶ感染的高危人群；ＨＧＶ常与ＨＢＶ或ＨＣＶ重叠／联合感染，也可单独感染；ＨＧＶ是非甲戊型肝炎的致病因子之一。抗ＨＧＶ阳性者中ＨＧＶＲＮＡ检出率为６６％（６４／９７），提示抗ＨＧＶＥＩＡ可用于ＨＧＶ感染的检测。

o investigate the prevelence of antiHGV antibody among different populations and patients with various liver diseases.Methods AntiHGV,HBsAg,antiHBs,antiHBc and antiHCV in sera were detected by enzymelinked immunoassays(EIA).AntiHGV positive sera were further tested for HGV RNA by a reverse transcription nested polymerase chain reaction (RTnPCR).The prevalence of antiHGV antibody in different populations and patients with various liver diseases were as folows:4/395(10%) in general population,10/278(36%) in blood donors,70/541(129%) in plasmas donor,22/162(136%) in intravenous drug addicts,12/56(214%) in hemodialysis patients,3/25(120%) in HBsAg carriers,11/40(262%) in chronic hepatitis B,17/136(125%) in chronic hepatitis C,4/11(364%) in cirrhosis and 3/16(188%) in chronic nonA to E hepatitis patients.Among 97 antiHGV positive individuals,64(66%) were HGV RNA positive,indicating the possibility of use of antiHGV EIA for the screening of blood donors to reduce posttransfusion HGV infection.