壬基酚对河川沙塘鳢性腺分化和发育影响的研究

Effect of Nonylphenol on the Gonadal Differentiation and Development in Gobbid Fish(Odontobutis potamophila)

利用组织学方法研究了环境雌激素化合物壬基酚(4-nonylphenol,NP)对河川沙塘鳢性腺分化和发育的影响。将人工孵化河川沙塘鳢仔鱼暴露于3个质量浓度(100、300、500μg/L)的NP中,同时设空白对照组和溶剂对照组,暴露150 d,显微观察性腺组织。试验结果表明,河川沙塘鳢性腺分化的过程是由中性的性腺直接分化出卵巢或精巢。暴露于500μg/L NP以下的各处理组中的河川沙塘鳢性别比例约为1∶1,与对照组无差异;但是精巢未发育的个体数量随NP浓度提高而增加,在NP 300μg/L和NP 500μg/L暴露组中分别占样本总数的20%和22%;此外,NP对精巢组织结构产生一定的伤害,精巢中出现坏死细胞和纤维化现象。

Larval gobbid fish Odontobutis potamophila （Gütinther）were randomly exposed to environmental estrogen compound,4-nonylphenol （NP）for 150 days at a rate of 0, 100, 300 and 500μg/L（nominal con- centrations）to evaluate effect of environmental estrogen compound NP on gonadal differentiation and development in the gobbid fish by histological techniques. The results showed that both ovary and testis were derived from undifferentiated gonads with sex ratio of 1 ： 1, without significant differences among the treatments. But the number of the fish with undeveloped testis was found to increase with increase in NP concentration. The fish with undeveloped testis accounted for 20% at 300 μg/L NP and 22% at 500μg/L NP. Furthermore, increased testicular fibrosis and necrotic testicular cells were observed in the fish exposed to the high concentration of NP.