正交试验法优选新藤黄酸的醇提工艺

Optimizing extraction process of neo-gambogic acid from gamboge by orthogonal test

目的优选藤黄中新藤黄酸的提取工艺。方法以新藤黄酸为考察指标,用HPLC法进行含量测定,采用正交试验法优化提取条件。结果最佳醇提条件为先以甲醇(pH=7)常温浸提,时间为20 min,再回流提取5 h,共2次。结论醇提的次数对新藤黄酸的提取影响最大,该研究确定的新藤黄酸的提取工艺合理、可行。

Aim To optimize the technology of extracting neo-gambogic acid from gamboge. Methods HPLC was used to analyze the content of neo-gambogic acid,and the orthogonal test was carried out to optimize the extraction conditions. Results The neo-gambogic acid was extracted at a room .temperature with methyl alcohol（ pH = 7）, then reflux 5 h twice. Conclusion The times of extraction greatly influenced the extraction of neo-gambogic acid, and the technology is feasible and reasonable.