β-环糊精对SOD的化学修饰

STUDY ON PHYSICOCHEMICAL PROPERTIES OF Cu,ZnSUPEROXIDE DISMUTASE MODIFIED WITH βCYCLODEXTRIN

牛血铜锌－超氧化物歧化酶（Ｃｕ，Ｚｎ－ＳＯＤ）用β－环糊精修饰后，其理化性质发生了部分变化：修饰后的酶呈不均一性，在聚丙烯酰胺凝胶电泳出现４条带，在薄层平板等电聚焦电泳中，等电点降低，修饰酶的紫外吸收光谱未见明显改变，抗蛋白酶水解能力增加。

Bovine Cu, Znsuperoxide dismutase was modified with βcyclodextrin(βCD) activated by NaIO4. As compared with that of native SOD, the physicochemical properties of the modified enzyme changed partly. The modified SOD was highly heterogeneous with at least 4 bands on the sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDSPAGE), and showed lower isoelectric point on the thin layer isoelectric focusing but no obvious change on the ultraviolet spectra. The modified SOD was more resistent to enzymic degradation.