N-乙酰半胱氨酸对糖尿病大鼠肾脏氧化应激的影响

The Effects of N-acetylcysteine on Oxidative Stress of the Kidneys of Diabetic Rats

目的:研究N-乙酰半胱氨酸(NAC)对糖尿病大鼠肾组织氧化应激的影响。方法:采用链脲佐菌素诱导糖尿病模型,以NAC(75mg.d-1)干预8周后,测定血糖、肾重/体重、尿白蛋白排泄率,并检测肾组织中丙二醛(MDA)含量及肾组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)的活性。结果:与正常对照组比较,模型组肾组织MDA含量显著增加(P<0.05),SOD、CAT和GSH-Px活性均显著降低(P<0.05);与模型组比较,NAC组血糖无显著差异(P>0.05),但肾重/体重和尿白蛋白排泄率显著降低(P<0.05),肾组织MDA含量显著降低(P<0.05),SOD、CAT和GSH-Px活性显著增加(P<0.05)。结论:糖尿病大鼠肾脏存在明显氧化应激反应,N-NAC能明显减弱肾脏氧化应激反应,进而起到保护肾脏和延缓糖尿病肾病发生、发展的作用。

Objective：To study the effects of N-acetylcysteine （NAC） on oxidative stress of the kidneys of diabetic rats. Methods：The diabetic model was induced by streptozotocin. The rats were divided into 3 groups： control group,diabetic group and diabetic rats receiving NAC. NAC was given at a dose of 75 mg · d ^-1 Blood glucose, body weight （BW）,kidney weight （KW）, ratio of kidney weight to body weight （KW/BW）, Urinary albumin excretion rate（UAER） were measured. The levels of malondialdehyde （MDA） in renal tissue as well as activities of superoxide dismutase （SOD）, catalase （CAT） and gtutathione peroxidase （GSH-Px） in renal tissue were determined. Results： Increased KW/BW, UAER were significantly attenuated by treatment with NAC（P〈0.05）. Elevated MDA level in diabetic renal tissue as well as decreased activities of SOD, CAT and GSH-Px in diabetic renal tissue were also remitted by NAC （P〈0.05）. Conclusion：This study shows that the kidneys of diabetic rats undergo an obvious oxidative stress. NAC treatment can to some extent protect kidneys from oxidative injury by diabetes.