子宫内膜癌雌激素受体α、β亚型和孕激素受体mRNA表达与临床病理的关系

Analysis of Estrogen Receptor α 、Estrogen Receptor β and Progesterone Receptor in Endometrial Carcinoma and Its Clinicopathologic Feature

目的:探讨子宫内膜癌雌激素受体亚型ERα、ERβ和孕激素受体(PR)与临床病理特征的关系。方法:应用半定量逆转录聚合酶链反应(RT-PCR)检测50例子宫内膜癌组织中的ERα、ERβ和PRmRNA的表达,并结合患者的临床病理资料进行综合分析。结果:①50例子宫内膜癌组织中,ERαmRNA阳性表达率为74%(37/50),平均相对表达量为0.5279±0.1295;ERβmRNA阳性表达率为48%(24/50),平均相对表达量为0.3025±0.1687;PRmRNA阳性表达率为94%(47/50),平均相对表达量为0.6983±0.2864;ERα的表达率和平均相对表达量均比ERβ高(P<0.01)。②子宫内膜样腺癌中的ERα、ERβmRNA的阳性表达率大于其他组织学类型(P<0.05),ERα和PRmRNA的相对表达量在子宫内膜样腺癌组织中均大于其他组织学类型(P<0.05)。③ERα和PRmRNA相对表达量随组织学分级的增高而降低(P<0.05);ERβmRNA则无此变化。④PRmRNA的相对表达量随手术病理分期的增高而显著降低(P<0.01),ERα和ERβmRNA的表达无此变化。结论:ERα、ERβ和PRmRNA在子宫内膜癌的异常表达与子宫内膜癌的组织学类型、组织学分级和手术病理分期有关,可作为子宫内膜癌的预后和内分泌治疗的参考。

Objective：To explore the relationship between the expression of estrogen receptor alpha （ERα）, estrogen receptor beta（ERβ）and progesterone receptor in endometrial carcinoma tissue and the clinicopathologic feature. Methods.. Using semiquantitive reverse transcription-polymerase chain reaction（RT-PCR）, we examined the expression of ERα, ERβ and PR mRNA in 50 cases of endometrial carcinoma tissues. Results： （1） ERα, ERβ and PR mRNA were detected in 74 %, 48 % and 94 % endometrial carcinoma,respectively. The average expression quantity of ERα, ERβ and PR mRNA in endometrial carcinoma was 0. 5279 ± 0. 1295, 0.3025 ± 0. 1687 and 0. 6983 ± 0. 2864,respectively. The expression of ERa mRNA in endometrial carcinoma tissues significantly higher than ERβ mRNA（P〈0.01 ）. （2）The positive rate of ERα and ERβ were higher in Endometrioid Adenocarcinoma than other histological subtypes（p〈0. 05）, respectively; While the average expression quantity of ERα and PR were higher in Endometrioid Adenocarcinoma than other histological subtypes（p〈0. 05）, respectively. （3） Higher ERα and PR mRNA expression significantly correlated with histological grade（p〈0.05）. （4） Higher PR mRNA expression significantly correlated with the clinical-surgical stage （p〈0.05）. Conclusion： The abnormal expression of ERα,ERβ and PR was closed related to histological subtype ,clinical-surgical stage and histological grade. Therefore, they maybe helpful for the diagnosis and endocrine therapy of Endometrial caocinoma.