紫外分光光度法测定国产注射用阿洛西林钠的含量

DETERMINATION OF SODIUM AZLOCILLINATE BY UV-SPECTROPHOTOMETRY

以１．２Ｍ／Ｌ的咪唑为催化剂，在含１０－３Ｍ／Ｌ氯化汞的中性溶液（ｐＨ６．８）中，于７０℃定量形成稳定的青霉烯酸硫醇汞盐，在３２５ｎｍ处有最大吸收峰．采用紫外分光光度法测定注射用阿洛西林钠的含量，考察了溶液的ｐＨ值、反应温度及时间对含量测定的影响，并与ＨＰＬＣ法（部颁标准）作了比较试验，ＲＳＤ＝０． ４７％，ｎ＝５；样品含量测定的平均 ＲＳＤ为 ０．８５％，ｎ＝６。与 ＨＰＬＣ法比较，无显著性差异。

Sodium azlocillinate could be deter- mined using UV spectrophotometry by measurement of its derivative,penicillenic acid mercuric mercaptide, at 325nm. This derivative was yeilded quantitatively by heating at 70 in 1.2mol/L imidaxole and 10-3mol/L mercuric chloride solution at pH6. 8. The precision of the method was 0.47%(RSD). The factors influencing the derivatizing reaction such as pH,time and tempera- ture were investigated.