环境胁迫对雷公藤中雷公藤红素含量的影响

EFFECTS OF ENVIRONMENTAL STRESSES ON CELASTROL CONTENTS IN TRIPTERYGIUM WILFORDII

雷公藤(Tripterygium wilfordii)是我国的名贵药用植物,其药用成分雷公藤红素是医药研究中重要的化学单体。环境胁迫能促进药用植物次生代谢物质的合成与积累,该文通过正交设计,探讨了水分、光照、土壤氮含量三因素两水平轻度胁迫处理对雷公藤幼苗中雷公藤红素含量的影响。研究结果表明:根部是雷公藤红素含量最丰富的部位;雷公藤幼苗在水分、光照及土壤氮含量胁迫条件下根系中雷公藤红素的含量分别提高了10.8%、14.4%和13.0%,光照对雷公藤红素含量的增加最明显,短期的轻度胁迫可提高根中雷公藤红素的含量。

Aims Tripterygium wilfordii is a highly valuable traditional Chinese medicinal plant. Celastrol, an active compound of T. wilfordii, has recently received attention from pharmaceutical researchers due to its medicinal value. Environmental stress may promote the formation and accumulation of secondary metabolites of medicinal plants. Our objective was to investigate whether mild environmental stress increased the content of Celastrol in T. wilfordii. Methods Orthogonal comparison of mild stress treatments of water, sunlight and N content were performed on the seedlings of T. wilfordii in the greenhouse. The content of Celastrol was determined by High Performance Liquid Chromatography （HPLC）. Important findings Celastrol was mainly produced in the seedling root and was increased 10.8%, 14.4% and 13.0% under the three different stress treatments, respectively. Therefore, mild stress treatment improves the synthesis and accumulation of Celastrol.