不同水平Gln对早期断奶犊牛免疫功能的影响

Effects of different injected glutamine levels on immune function in early-weaned calves

选用体质量和出生日期相近的12头中国荷斯坦犊牛,随机分为4组,(40±1)d一次性断奶。采用胃肠外营养(Total parenteral nutrition,TPN)颈静脉灌注丙氨酰谷氨酰胺(Ala-Gln)二肽的方式,研究了不同水平Gln对早期断奶犊牛免疫功能的影响。Ala-Gln灌注剂量为Ⅰ组(对照组,不灌注),Ⅱ组(低水平)0.33 g/(kg.d),Ⅲ组(高水平)1.34 g/(kg.d),Ⅳ组(中等水平)0.68 g/(kg.d),连续灌注7 d。结果表明:补充中等水平的Ala-Gln,可以有效地增加外周血CD2+和CD4+淋巴细胞的含量,使CD4+/CD8+比值增高,同时使血清IgA和IgG含量和空肠和回肠粘液S-IgA含量显著升高,从而增强了机体的免疫功能。

Twelve Chinese Holstein calves with similar weight and date of birth were selected and divided randomly into four groups. They were weaned at （40± 1） d. Alanyl-glutamine peptide （Ala-Gln） was supplemented through jugular vein by total parenteral nutrition （TPN） to explore the effects of different injected glutamine levels on immune function in early-weaned calves. Ala-Gln levels were designed as follows：trial Ⅰ（control group,0 g/（kg · d）,trail Ⅱ （low-Gin group,0.33 g/（kg·d）,trail Ⅲ （high-Gin group,1.34 g/（kg· d）,trail IV （mid-Gln group, 0.68 g/（kg· d） ,and the injection lasted for 7 d continuously. The results indicated that mid-Gln level could increase effectively peripheral blood＇s level of CD2^＋ and CD4^＋ lymphocyte and the ratio of CD4^＋/CD8^＋. At the same time, the levels of serum IgA, IgG and S-IgA in jejunum and ileum mucus increased significantly resulting in improvement of animals＇ immune function.