褪黑素对内毒素血症山羊肝功能损伤的影响

Effect of melatonin on liver impaired in goat with endotoxemia

4月龄山羊24只,随机分成4组,每组6只,即对照组(生理盐水,NS),内毒素血症组(脂多糖LPS 1 mg/kg),内毒素血症褪黑素组(褪黑素MT 1 mg/kg),褪黑素组。在注射后0.5、1、2、3、4、5、6 h采血,测定不同组别及不同时间段血清中ALT、AST、ALP、CP、TP、ALB值。结果:LPS组血清中的ALT、AST、ALP活性明显高于NS组;LPS+MT组ALT、AST、ALP酶活性也有所升高,但至6 h时基本恢复到NS组的水平,而MT组中的各项指标与NS组差异不显著(P>0.05)。LPS组血清CP、TP、ALB值明显低于NS组;LPS+MT组与NS组无显著差异。结果表明MT可减缓内毒素对肝脏的损伤作用。

To study the effect and mechanism of melatonin on hepatic injure by endotoxin in goats. Twenty-two hybrid goats were divided randomly into 4 groups：group I treated with the same volume saline; group Ⅱ received 5 ml saline containing lipopolysaccharide （LPS） （E. coli O111 B4 ）at 1 mg/kg body weight;group Ⅲ received melatonin （MT） at 1 mg/kg body weight before injection of the same dose of LPS;group IV received 5ml saline containing MT for 1 mg/kg body weight. The serum was collected from jugular vein at 0. 5,1,2,3,4,5,6 h after injection respectively. The activities of glutamate pyruvate transaminase（ALT）, glutamic-oxaloacetic transaminase （AST）, alkaline phosphatase （ALP）, contents of ceruloplasmin（CP）, total protein（TP） and albumen（ALB） were examined for evaluating the liver function. The activities of ALT,AST and ALP increased significantly in endotoxin-treated goats compared to saline-treated controls （P〈0.05）;The activities of ALT, AST and ALP ingoats treated with LPS＋ MT were nearly similar to goats treated with saline-treated controls at 6 h（P〉0.05）; The CP, TP and ALB contents decreased significantly in endotoxin-treated goats compared with the control group （P〈0.05）： the contents of CP,TP and ALB in goats treated with LPS＋ MT were nearly same as goats treated with saline-treated controls （P 〉0. 055. MT play an important role in protecting the liver structure and function in goat with endotoxemia.