能量对初情期前母猪卵巢LHR和FSHR mRNA表达的影响(英文)

Effects of dietary energy level on ovarian expression of mRNA s for luteinizing hormone receptor and follicle-stimulatiing hormone receptor in prepubertal gilts

对9头50日龄、体质量为30 kg的初情期前杜×长×大三元杂交母猪进行长期日粮能量差异饲养试验。饲养结束后屠宰,取卵巢液氮保存。半定量RT-PCR检测每头母猪卵巢LH和FSH受体mRNA的含量。结果,高能组卵巢LH和FSH受体mRNA的含量显著高于中能组和低能组(P<0.05);而低能组显著低于中能组和高能组(P<0.05)。表明,能量水平高的日粮可以显著地促进初情期前卵巢上LH和FSH受体在mRNA水平的表达,而能量水平不足的日粮则不利于这种表达。本文首次报道了日粮能量水平对母猪卵巢LH和FSH受体mRNA表达的影响。

To explore the effects of long-term treatment of different dietary energy levels on ovarian expression of mRNAs for LHR and FSHR, the present study was performed in nine growth-matched littermate crossbred （Landrace × Large White × Duroc） prepubertal gilts. At approximately 30 kg of body weight and 50 day of age,gilts were housed with individual feeding stalls and placed on a normal level of feeding for 90 days （dl-90） with free access to water and food throughout the whole research. From d91,littermates were divided and randomly assigned to one of the following three treatment lines for 3 weeks till d112 ..Group H, Group M,and Group L, fed the high energy level diet （n = 3, digestible energy 14.87 MJ/kg）, moderate energy level diet （n = 3, digestible energy 12. 39 MJ/ kg）,and low energy level diet （n = 3, digestible energy 9.98 MJ/kg）, respectively. When gilts were slaughtered on dl12 after 3 weeks energy treatment, both ovaries of every gilts were collected, snap frozen in liquid nitrogen and retained at -80℃ for use to determine and analysis the relative amount of ovarian LHR and FSHR mRNAs using semi-quantitative RT-PCR. Results showed that the effect of dietary energy treatment was notable., the ovarian expression of mRNAs for LHR and FSHR was significantly higher （P〈0.05） in gilts treated with high energy diet compared to gilts treated with moderate and low energy diets,while gilts treated with low energy diet had a significantly lower （P〈0.05） ovarian LHR and FSHR expression compared with gilts treated with moderate and high energy diets. These results revealed that ovarian expression of LHR and FSHR in prepubertal gilts increased as the level of dietary energy intake elevated,i, e. , high energy diet can markedly enhance ovarian expression of mRNAs for LHR and FSHR,whereas energy deficit markedly suppress the expression.