两种不同选择标记的转化效率比较

Comparison of transformation efficiency between selective markers hpt and bar

为了弄清两个选择标记在相同条件下的转化效率,为转基因选择遗传标记时提供参考,以优良恢复系明恢63为受体材料,采用农杆菌介导的遗传转化法,分别以hpt和bar基因为选择标记进行了转化.结果显示:1 500块胚性愈伤共培养后获得214块抗性愈伤,转化效率为14.3%;5 400块胚性愈伤共培养后获得156块抗性愈伤,转化效率为2.9%;两者的分化率分别为79.0%和80.7%.此外,以hpt和bar为选择标记,从胚性愈伤诱导到获得转基因植株分别需要75天和125天.因此,以hpt为选择标记的遗传转化,比较适合基因功能验证和共培养培育无选择标记水稻植株;如果是培育商品化的转基因水稻植株,则最好以bar为选择标记基因.

hpt and bar genes were mostly used as selectable markers in rice transformation. In order to understand the transformation efficiency with these two markers under the same condition for references,in the study, elite restorer MH63 was used as a receptor material to be transformed via Agrobacterim with hpt and bar as selective marker, respectively. Results displayed that 214 and 156 of resistant calli were obtained from 1 500 and 5 400 calli, respectively and their transformation efficiencies were 14. 3% and 2. 9%, respectively. Their differentiation efficiencies have no obvious difference. Besides, it took 75 days produce transgenic plantlets from calli using hpt as selectable marker and 125 days using bar as marker. Therefore, hpt is suitable for the culture of marker-free transgenic plants or confirmation of gene function, while bar is suitable for culture of commercialized transgenic plants.