摘要
目的监测孕妇产前感染HBV与宫内感染情况,评估乙肝孕妇血清中乙肝血清标志物(HBVM)对宫内感染HBV的风险大小,指导育龄妇女早期预防宫内感染。方法采用ELISA法检测孕妇血清乙肝标志物(HBVM)6项,即HBsAg、HBsAb、HBeAg、HBeAb、HBcAb与Pre-S1;筛选乙肝携带者采用实时荧光定量PCR法进行HBV DNA检测;应用ELISA法对新生儿24h内(免疫接种前)外周血HBsAg、Pre-S1和`HBeAg进行检测。结果1002例孕妇中乙肝感染率为10.6%,106例乙肝孕妇宫内感染34例,宫内感染率为32%。HBeAg(+)者宫内感染风险率最高为77%,HBV DNA(+)与Pre-S1(+)次之。HBV DNA(-)宫内感染风险率最低为2%,HBeAg(-)与Pre-S1(-)次之。结论乙肝孕妇宫内感染率较高;HBeAg(+)孕妇宫内感染HBV风险最高,可作为育龄妇女采取措施积极预防宫内感染的指标。HBV DNA(-)宫内感染率最低,可作为育龄妇女宫内安全的评估指标。Pre-S1是宫内感染的有利补充指标。
Objective To explore HBV infection of pregnant women monitor and evaluate intrauterine infection with HBV markers. Method Serum HBV markers were determined by ELISA, and HBV DNA was determined by FQ- PCR. Result HBV infection rate was 10.6% in pregnant women. HBV intrauterine infection was 32% in HBV pregnant women. Conclusion The rate of HBV intrauterine infection was high. HBV DNA Negative and HBeAg Positive have a diagnostic value in HBV intrauterine infection.
出处
《中国微生态学杂志》
CAS
CSCD
2009年第1期52-54,共3页
Chinese Journal of Microecology
基金
重庆市十一五科技攻关重点资助项目(NO.0200060252)
