期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNA干扰沉默COX-2对鼻咽癌细胞增殖、凋亡及细胞周期的影响 被引量：5

下载PDF
导出
摘要 目的筛选COX-2基因的有效RNA干扰序列,研究沉默COX-2表达对鼻咽癌细胞增殖、凋亡以及细胞周期的影响。方法人工合成双链的小干扰RNA(siRNA),脂质体法转染鼻咽癌C666-1细胞后,RT-PCR法检测COX-2的mRNA含量表达变化,筛选出沉默COX-2表达的有效RNA干扰序列。MTT法检测细胞增殖的变化,DAPI染色检测细胞凋亡,流式细胞法进行细胞周期分析。结果设计合成的anti-COX-2b siRNA能够使COX-2mRNA表达下降90%以上,COX-2基因沉默后,鼻咽癌细胞周期出现G0/G1期阻滞,增殖能力下降,但对凋亡无明显影响。结论RNA干扰沉默COX-2的表达能够抑制细胞增殖,阻滞细胞周期,可以成为鼻咽癌研究和抗肿瘤基因治疗提供新的思路,进行深入研究。
作者 李刚 李湘平 陈顺金 鲁娟 王路
机构地区 南方医科大学南方医院耳鼻喉科
出处 《广东医学》 CAS CSCD 北大核心 2009年第1期45-48,共4页 Guangdong Medical Journal
关键词 鼻咽肿瘤 环氧合酶-2 RNA干扰
分类号 R711.71 [医药卫生—妇产科学] R733.1 [医药卫生—肿瘤]
  • 相关文献

参考文献11

  • 1OHNO S, OHNO Y, SUZUKI N, et al. Multiple roles of cyclooxygenase - 2 in endometrial cancer [ J ]. Anticancer Res, 2005, 25 (6A) : 3 679 -3 687.
  • 2CHEN Wen - cheng, McBRIDE W H, CHEN Shih - ming, et al. Prediction of poor survival by cyclooxygenase - 2 in patients with T4 nasopharyngeal cancer treated by radiation therapy: clinical and in vitro studies[J]. Head Neck, 2005, 27(6) : 503 -512.
  • 3LI Xiang - ping, LI Gang, PENG Ying, et al. Suppressin of Epstein - Barr virus - encoded latent membranee protein - 1 by RNA interference inhibits the metastatic potential of nasopharyngeal carcinoma cells [ J ]. Biochem Biophys Res Commun, 2004, 315 (1): 212-218.
  • 4SOO R, PUTTI T, TAO Q, et al. Overexpression of cyclooxygenase -2 in nasopharyngeal carcinoma and association with epidermal growth factor receptor expression [ J ]. Arch Otolaryngol Head Neck Surg, 2005, 131(2): 147-152.
  • 5MURONO S, INOUE H, TANABE T, et al. Induction of cyclooxygenase - 2 by Epstein - Barr virus latent membrane protein 1 is involved in vascular endothelial growth factor production in nasopharyngeal carcinoma cells[J]. Proc Natl Acad Sci, 2001,98(12) : 6 905 -6 910.
  • 6TAN K B, PUTFI T C. Cyclooxygenase 2 expression in nasopharyngeal carcinoma: immunohistochemical findings and potential implications[ J ]. J Clin Pathol, 2005, 58 (5) : 535 - 538.
  • 7PENG Jyh - ping, CHANG Hui - chiu, HWANG Chung - feng, et al. Overexpression of cyclooxygenase - 2 in nasopharyngeal carcinoma and asseciation with lymph node metastasis[ J]. Oral Oncol, 2005, 41(9) : 903 -908.
  • 8AMIR M, AGARWAL H K. Role of COX -2 selective inhibitors for prevention aad treatment of cancer[ J]. Pharmazie, 2005, 60 ( 8 ) : 563 - 570.
  • 9何美蓉,林劲秋,宋于刚.选择性COX-2抑制剂对大鼠胃溃疡微血管形成的影响及机制[J].广东医学,2008,29(3):379-381. 被引量：3
  • 10李刚.RNAi研究现状与进展[J].广东医学,2003,24(3):327-329. 被引量：5

二级参考文献20

  • 1[1]Wassenegger M. Gene silencing. Int Rev Cytol, 2002,219:61
  • 2[2]Fire A, Xu S, Montgomery MK, et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature,1998,391:806
  • 3[3]Zamore PD, RNA interference: listening to the sound of silence. Nat Struct Biol, 2001 ,8(9):746
  • 4[4]Dalmay T, Hamilton A, Rudd S, et al. An RNA-dependent RNA polymerase gene in Arabidopsis is required for post-transcriptional gene silencing mediated by a transgene but not by a virus. Cell, 2000,101:543
  • 5[5]Harborth J, Elbashir SM, Bechert K, et al. Identification of essential genes in cultured mammalian cells using small interfering RNAs.J Cell Science,2001,114(24):4557
  • 6[6]Miyagishi M, Taira K. U6 promoter-driven siRNAs with four uridine 3' overhangs efficiently suppress targeted gene expression in mammalian cells. Nature Biotechnology, 2002, 19:497
  • 7[7]Brummelkamp TR, Bernards R, Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science, 2002,296:550
  • 8[8]Sui GS, Soohoo C, Affar EB. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. PNAS, 2002,99(8):5515
  • 9[9]Lee NS, Dohjima T, Bauer G, et al. Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nature Biotechnology,2002,19:500
  • 10[10]McCaffrey AP, Meuse L,Pham TT, et al. RNA interference in adult mice. Nature, 2002,418:38

共引文献6

同被引文献59

  • 1成南南,杨萌哲,杨建宇,吕安乔,白先愚,郭兴喆,黄元姣.基于网络药理学探讨野菊花治疗鼻咽癌的可能作用机制[J].基因组学与应用生物学,2021,40(7):2825-2836. 被引量：6
  • 2李虹,韩为农,张玲,冯湘玲,姚开泰.POSSIBLE REASONS FOR TP53 ACCUMULATION IN NASO-PHARYNGEAL CARCINOMA USING ATLAS HUMAN CANCER cDNA EXPRESSION ARRAY[J].Chinese Journal of Cancer Research,2002,14(1):28-32. 被引量：1
  • 3姜武忠,卢景琛,文继舫,廖遇平,张静,朱红,冯德云,沈明.鼻咽癌组织中nm23-H_1和survivin蛋白表达及其临床意义[J].中华放射肿瘤学杂志,2005,14(5):383-387. 被引量：7
  • 4孙晓菊.环氧合酶-2与口腔鳞状细胞癌关系的初步研究[D]中国医科大学,中国医科大学2005.
  • 5R. E. Harris.Cyclooxygenase-2 (cox-2) blockade in the chemoprevention of cancers of the colon, breast, prostate, and lung[J]. Inflammopharmacology . 2009 (2)
  • 6Jutta Renkonen,Henrik Wolff,Timo Paavonen.Expression of cyclo-oxygenase-2 in human tongue carcinoma and its precursor lesions[J]. Virchows Archiv . 2002 (6)
  • 7H. Inoue,M. Takamori,N. Nagata,T. Nishikawa,H. Oda,S. Yamamoto,Y. Koshihara.An investigation of cell proliferation and soluble mediators induced by interleukin 1β in human synovial fibroblasts: comparative response in osteoarthritis and rheumatoid arthritis[J]. Inflammation Research . 2001 (2)
  • 8CY Yang,CL Meng,CL Liao,PY Wong.Regulation of cell growth by selective COX-2 inhibitors in oral carcinoma cell lines. Prostaglandins and Other Lipid Mediators . 2003
  • 9Chandrasekharan N V,Dai H,Roos K L,et al.COX-3, a cyclooxygenase-1 variant inhibited by acetaminophen and other analgesic/antipyretic drugs: cloning, structure, and expression. Proceedings of the National Academy of Sciences of the United States of America . 2002
  • 10Appleby SB,Ristimaki A,Neilson,et al.Structrue of the human cyclooxygenase-2 gene. Biochemical Journal . 1994

引证文献5

二级引证文献10

广东医学
2009年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部