穿心莲提取物体外抗菌活性及稳定性的研究

Studies on Bacteriostatic Activity of Andrographis paniculata Extracts and its Stability

采用杯碟法对穿心莲提取物进行体外抗菌作用以及pH、温度因素对其抗菌活性的影响。结果表明：穿心莲提取物对金色葡萄球菌、枯草杆菌、大肠杆菌、黑曲霉、青霉都有明显的抑菌效果，最低抑菌浓度分别为0.5,0.25、0.25、0.125、0.25mg／mL；最小杀菌浓度为0.5,0.5,0.5,0.5、0.25mg／mL。穿心莲提取物的抑菌活性对热稳定性较差，在pH4-7的条件下抑菌效果最佳。

In this paper,bacteriostatic activity of Andrographis paniculata extracts and the effect of temperature, pH value on its antibacterial activities were studied by cylinder plate method. The results showed Andrographis paniculata extracts had strong inhibition effects on Staphylococcus aureus, Bacillus subtil, Esckerichia coli, Aspergillus niger and Penicilliurn, the minimum inhibitory concentration （MIC） was 0. 5, 0. 25, 0.25, 0. 125, 0. 25 mg/mL, and minimum bactericidal concentrations（MBC） was 0. 5, 0.5, 0.5, 0.5, 0.25 mg/mL respectively. The antibacterial activity of Andrographis paniculata extracts was unstable under heating,and the antibacterial effects was best at pH 4-7.