外周血单核细胞γ干扰素分泌反应在免疫抑制患者肺结核诊断中的应用

Application of γ-interferon secretory reaction in peripheral blood monocytes in diagnosis of tuberculosis in patients with immunosuppression

目的:探讨体外单核细胞γ干扰素(IFN-γ)分泌反应对免疫抑制患者肺结核诊断的临床意义。方法:应用ELISPOT的方法检测了12名健康体检者(健康对照组)、12名结核病患者(结核病对照组)和12名免疫抑制合并结核病患者(免疫抑制合并结核病组)体外单核细胞IFN-γ分泌反应。结果:健康对照组中除1人有结核病接触史者,IFN-γ分泌反应呈阳性外,其余均为阴性;结核病对照组中除1名患者痰培养证实为非结核分枝杆菌,其IFN-γ分泌反应呈阴性外,其余均为阳性;免疫抑制合并结核病患者均为阳性。结论:体外单核细胞IFN-γ分泌反应有助于临床对免疫抑制患者合并肺结核的诊断。

Objective To investigate the clinical significance of γ-interferon （IFN-γ） secretory reaction in monocytes in vitro in the diagnosis of tuberculosis in patients with immunosuppression. Methods IFN-γ secretory reactions in monocytes in vitro were detected by enzyme-linked immunospot test in 12 healthy subjects, 12 tuberculosis controls and 12 immunosuppressive patients with tuberculosis. Results IFN-γ secretory reaction was negative in healthy group except one subject with contact history of tuberculosis, was positive in tuberculosis controls group except one patient who was proved through sputum culture to be infected by anonymous mycobacteria, and was positive in immunosuppressive patients with tuberculosis. Conclusion Detection of IFN-γ secretory reaction in monocytes in vitro is conducive to the diagnosis of tuberculosis in patients with immunosuppression.