河北省卢龙县1999～2005年婴幼儿杯状病毒腹泻的监测研究

Epidemiology on caliciviruses diarrhea of children in Lulong county of Hebei province in 1999～2005.

【目的】研究河北省卢龙县5岁以下婴幼儿杯状病毒腹泻的流行病学特征。【方法】应用酶联免疫吸附试验（ELISA）和逆转录一聚合酶链反应（RT—PCR）方法对1999-2005年间卢龙县医院和妇幼保健院住院的腹泻病人检测杯状病毒（HUCVs）并进行流行病学调查。【结果11075例腹泻病人的粪便样本HUCVs检测，用ELISA方法检测阳性率为38．6％，RT—PCR方法检测阳性率为25．7％，两种方法均阳性为21．2%。婴幼儿杯状病毒腹泻的年龄分布：以12～17月龄组发病最多，其次为6～8月龄，9～11月龄组和3～5月龄组。季节分布：以历年1月份为发病高峰，其次是12月和2月份。【结论】河北省卢龙县杯状病毒婴幼儿腹泻发病严重程度仅次于轮状病毒腹泻，流行病学特征与轮状病毒腹泻极为相似。

[Objective] To study the epidemiological characterstus of caliciviruse diarrhea among children aged 0-5 years old. [Method] Stool specimens collected from disarrhea patients were tested for human calicivirus by enzyme linked immunoassy （ELISA） and revere-transcription polymerase chain reaction （RT-PCR） . [ Results] Caliciviruse was detected in 1 075 tools specimens of diarrhea children, the positive rate were 38.6%, 25.7%, 21.2% by ELISA, RT- PCR and both two methods above respectively. Children aged 12-17 months were most easy to had caliciviruse diarrhea, then the children aged 6-8 months, 9-11 months and 3-5 months. [Conclusion] Human ealieivirus was confirmed to be the common cause of the severe diarrhea, the epidemiologic feature is similar to Rotavirus.