难处理金矿吸附浸矿细菌多糖含量变化的研究

Study on Change of Polysaccharide Content in Leaching Bacteria During Adsorbing Refractory Gold Ore

采用苯酚-硫酸方法测定HQ0211菌裂解液中多糖含量，得出最佳的裂解时间为25min，最佳吸收波长为430nm，最佳冷却时间为10min．在利用HQ0211菌对含砷金矿进行吸附氧化试验研究时，微生物细胞多糖含量随着溶液体系电位的变化而变化．在480-572mV阶段，游离在溶液中的细菌的多糖质量浓度为0．033-0．123mg／mL，吸附在含砷金矿石矿物上的细菌细胞多糖为5．3～8．9mg／g；当电位达到638mV时，溶液中游离的细菌细胞多糖质量浓度达1．155mg／mL，吸附在矿物上的细菌细胞多糖可高达57．5mg／g．

The polysaccharide content in the bacterium HQ0211 lysate was determined by phenolvitriol method, from which some optimal values were given including the lyric time 25 min, adsorption wavelength 430 nm and cooling time 10 min. In the test using the leaching bacterium HQ0211 to adsorb/oxidate the refractory As-bearing gold ore, it was found that the polysaccharide of bacterium cell changes with the potential of solution. When the potential is 480 -572 mV and 638 mV, the polysaccharide contents of free bacterial cells in solution and of bacterial cells adsorbed on grains of As-bearing gold ore are 0. 033--0. 123 mg/mL and 5.3--8.9 mg/g, 1. 155 mg/mL and 57.5 mg/g, respectively.