烟草和MPTA对三孢布拉氏霉合成番茄红素的影响

The application of tobacco and MPTA during lycopene production by Blakeslea trispora

在三孢布拉氏霉发酵48h添加3.6mmol/L2-(4-甲基苯氧)-三乙基胺(MPTA)可有效抑制96%β-胡萝卜素的合成;添加15g/L烟草可促进总类胡萝卜素的产量(170mg/L),但其对β-胡萝卜素的合成抑制作用低于MP-TA。L27(313)正交实验从抑制剂添加时间、添加量以及两种抑制剂的交互作用等方面考察了同时添加MPTA和烟草对番茄红素产量的影响。发酵48h同时添加烟草10g/L与MPTA2.2mmol/L,番茄红素的产量可达205mg/L(占总类胡萝卜素产量的94%)。

The efficacy and optimization of the application of two inhibitors, tobacco and bleaching herbicide 2- （4-methylphenoxy）-triethylamine （MPTA）, on lycopene productivity by Blakeslea trispora were investigated. The greatest yield of lycopene （170 mg/L） was observed when nicotine in the form of tobacco was added at 15 g/ L at 48 hours old mated cultures, however, this addition did not inhibit （-carotene synthesis to the same extent as MPTA＇ s, but resulted in 53 % increase in overall carotenoid synthesis. Lycopene biosynthesis was strongly stimulated （205mg/L） at the expanse of （-carotene when 2.2mmol/L MPTA and 10g/L tobacco added together at 48 hours following inoculation. Because of the low quantity but great efficiency, this compound inhibitor of tobacco and MPTA will become a potential choice of inhibiting lycopene cyclase in Blakeslea trispora.