eNOS基因第四内含子a/b多态与湖北汉族人原发性高血压而不是2型糖尿病相关联

The a/b Polymorphism in the eNOS Gene is Associated with Essential Hypertension but not Type 2 Diabetes in Hubei Han Chinese

目的:探讨内皮型一氧化氮合酶基因(eNOS)与湖北汉族人原发性高血压(EH)和2型糖尿病(T2DM)的关系。方法:采用病例-对照设计,分析了657例样本eNOS第四内含子重复序列多态性a/b,测量了身高、体重、腰围、臀围、收缩压、舒张压、空腹血糖,餐后2小时血糖等临床指标。结果:EH病例组eNOS ab+aa基因型和a等位基因频率显著高于EH对照组(基因型:25.3%vs18.9%,P=0.049;等位基因:13.3%vs9.8%,P=0.045);而T2DM病例组与T2DM对照组的eNOS ab+aa基因型频率没有显著差异(20.2%vs24.1%,P=0.247)。单因素Logistic回归分析显示eNOS ab+aa基因型是EH的危险因子(OR=1.623,95%CI1.053-2.506,P=0.029)。多因素回归分析显示,EH的独立风险因素是年龄、体重指数和eNOS基因a/b多态性,而体重指数和腰臀比是T2DM的独立风险因素。结论:eNOS基因a/b多态性是湖北汉族人群EH的一个易感标记,而与T2DM没有相关性。

Objective： To explore the role of the endothelial nitric oxide synthase （eNOS） gene in essential hypertension and type 2 diabetes in Hubei Han Chinese. Method： 657 Chinese subjects from Hubei province were genotyped for the eNOS a/b polymorphism by unrelated case-control method, and their height, weight, waist circumference, hip circumference, systolic blood pressure （SBP）, diastolic blood pressure （DBP）, fasting plasma glucose （FPG）, postprandial plasma glucose （PPG） were measured. Results： Frequencies for eNOS ab＋aa genotype and a allele in essential hypertension patients were significantly higher than those in controls （genotype： 25.3% vs 18.9%, P=0.049; allele： 13.3% vs 9.8%, P =0.045）; Logistic regression analysis suggested that the eNOS ab＋aa genotype was the genetic risk factors for essential hypertension （OR=1.623, 95% CI 1.053-2.506, P=-0.029）. The eNOS a/b polymorphism was not associated with type 2 diabetes in our population. Multivariate logistic regression analysis indicated that risk factors for essential hypertension were the age, body mass index and eNOS a/b polymorphism, whereas body mass index and waist to hip ratio were risk factors for type 2 diabetes. Conclusion： The eNOS a/b polymorphism was associated with risk of essential hypertension, not type 2 diabetes in Hubei Han Chinese.