冠心病患者脂蛋白相关磷脂酶A2基因多态性研究

Connecting research to lipoprotein-associated phospholipaseA2 gene polymorphisms of the patients with coronary heart disease

目的探讨脂蛋白相关磷脂酶A2基因R92H和A379V多态性位点与冠心病遗传易感性的关系。方法采用单荧光标记探针技术检测261例冠心病患者、263例正常对照者脂蛋白相关磷脂酶A2基因R92H和A379V基因型。结果（1）92H等位基因携带者患冠心病的风险是92R等位基因携带者的1．75倍（P〈0．01）；（2）冠心病组中单倍型379A92H的频率分布显着高于对照组（P〈0．01）；（3）Logistic回归分析提示，R92H位点等位基因92H是冠心病的独立危险因素。结论脂蛋白相关磷脂酶A2基因R92H多态性与汉族人冠心病遗传易感性独立相关。

Objectives To investigate the association between R92H and A379V point mutation of lipoprotein-associated phospholipaseA2 （Lp-PLA2） and the patients with coronary heart disease, Methods 261 CHD patients and 263 normal controls were genotyped by using single-labeled probe technique. Results Compared with RR genotype, the relative risk for CHD in people with RH genotypes was 1.77 （ P 〈0.01 ）. The relative risk for CHD in people with 92R allele was 1.75 higher than 92H allele （P 〈 0.01 ）. The two polyn, orphisms of the LP-PLA2 gene were in strong linkage disequilibrium （LD）. The frequency of the haplotype 379A92H was significantly （P 〈 0.01 ） higher in CHD patients than normal controls. When allied-gene analysis was performed for RR and AV genotype ,the risk of coronary heart disease was markedly reduced 46% （0R = 0.54）, Binamy logistic regression analysis showed that polymorphism of R92H was an independent risk factor for CHD. Conclusion The polymorphism of R92H of Lp-PLA2 gene could be associated with risk of CHD in Han Chinese.