二氧化硫污染对运动大鼠血清和肺组织IL-6、TNF-α水平的影响

Effect of Sulfur Dioxide Pollution on IL-6 and TNF-α Level in Serum and Lungs in Exercising Rats

目的:探讨不同浓度SO2污染对运动大鼠血清和肺组织白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)水平的影响,从细胞因子的角度揭示SO2污染对运动大鼠毒作用的免疫学机制。方法:48只SD大鼠随机分为空白对照组(SG)、单纯运动组(EG)、高污染运动组(HEG)、高污染安静组(HSG)、中污染运动组(MEG)、中污染安静组(MSG)、低污染运动组(LEG)和低污染安静组(LSG),每组6只。除SG和EG外,其余各组置于不同浓度SO2污染环境中(5mg/m3,10mg/m3,15mg/m3),运动组大鼠进行跑轮运动(8m/min,2h/天,共10天)。采用放射免疫法检测血清和肺组织IL-6、TNF-α含量。结果:EG大鼠血清和肺组织IL-6水平较SG显著升高(P<0.05);HEG、MEG、LEG大鼠血清及肺组织IL-6、TNF-α均较SG显著升高(P<0.01),且HEG、MEG大鼠IL-6和TNF-α较HSG、MSG上升更明显(P<0.01,P<0.05),LEG大鼠肺组织IL-6和TNF-α较LSG显著升高(P<0.05)。结论:SO2污染环境中运动可引起体内致炎细胞因子高表达,低浓度SO2污染下运动对肺组织的致炎作用较血清更明显。

Objective To investigate the effect of sulfur dioxide （SO2） pollution on interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） level in serum and lungs of exercising rat. Methods Forty eight SD rats were randomly divided into eight groups： sedentary group （SG）, exercise group （EG）, exercise in high pollution group （HEG）, high pollution sedentary group （HSG）, exercise in moderate pollution group （MEG）, moderate pollution sedentary group （MSG）, exercise in low pollution group （LEG） and low pollution sedentary group （LSG）. All groups inhaled the various concentrations of SO2 （0mg/m^3 , 5mg/m^3 , 10mg/m^3 , 15mg/m^3 ）, and the exercise groups were exercising with a motor-driven wheel （8m/min, 2h/d for 10d）. The level of IL-6 and TNF-α in serum and lungs were measured by radioimmunoassay. Results As compared with SG, the IL-6 levql in serum and lungs increased in EG （P〈0.05）. The IL-6 and TNF-α levels in serum and lungs in HEG and MEG were significantly increased as compared with HSG and MSG （P〈0.01, P〈0.05）. As compared with LSG, the IL-6 and TNF-α levels in LEG increased in lungs （P〈0.05） without significant increase in serum （P〈0.05）. Conclusions In SO2 environment, exercising rats were more vulnerable to proinflammatory cytokine, especially their lung tissue.