小麦PEBP-like基因等位变异与籽粒大小、粒重关系研究

Study on Relationship between Allelic Variation in PEBP-like Gene and Grain Size and Weight in Common Wheat

小麦PEBP-like基因与籽粒大小、粒重相关。本文根据水稻GS3基因序列搜索小麦及其近源种属EST序列,电子克隆小麦PEBP-like基因,并设计特异引物在万县百麦子/京411构建的重组自交系群体(recomb inant inbredl ines,RILs)鉴定该基因变异与籽粒大小及粒重的关系。结果表明,本文小麦籽粒大小与粒重相关性较好,其中粒宽和粒重的相关性最好(0.668**),其次是粒厚(0.629**),粒长也与粒重呈极显著正相关(0.485**)。WKS3-8引物在亲本和群体中多态性较好,具有A型等位基因的家系,籽粒较小、粒重低,其大部分家系千粒重低于平均值(38.3g);而具有B型等位基因的家系,籽粒较大,粒重高,其大部分家系粒重高于平均值。上述说明PEBP-like基因等位变异与小麦粒长、粒宽、粒厚及粒重关系密切。

PEBP-like gene is associated with grain size and weight in common wheat. In our study, the gene was assembled with two wheat ESTs and three barley ESTs screened based on the GS3 sequence of rice in GenBank. The relationship between allelic variation of PEBP-like and wheat kernel size and weight was researched through PCR using a pair of primers designed as the assemble sequence. The results were indicated that good correlation was found between grain length, width, thickness and weight. Grain width behaved the best positive correlation with thousand grain weight （0.668^＊＊）, the next was grain thickness （0.629^＊＊） and grain length （0.485^＊＊）. Lines of RILs holding A type allele of PEBP-like showed smaller kernel and lower weight, on the contrary, lines with B type allele mean larger size and higher weight, which most of the lines were distributed the range of higher weight. Our results confirmed a close relation occurred in variation ofPEBP-like gene and kernel size and weight of common wheat.