小麦穗发芽抗性分子标记的有效性检测与验证

Detection and Validation of Molecular Marker Associated with Pre-Harvest Sprouting Resistance in Chinese Wheat

小麦收获前穗发芽严重影响加工品质,来年种用价值以及产量。本研究利用万县白麦子/京411穗发芽重组自交系群体对已报道的第3染色体上有关穗发芽抗性分子标记XBarc321、XBarc310和XBarc57以及第4染色体上Xbarc170、Xgwm397和Xgwm269进行有效性检测,并在穗发芽抗性不同的40份地方品种以及推广品种中进一步验证,结果表明,小麦第3染色体上的分子标记XBarc321、XBarc310和XBarc57在穗发芽群体以及穗发芽抗性不同的品种中选择效应较大,尤其是XBarc321和XBarc310,证实该标记与穗发芽抗性紧密相关,而第4染色体上的分子标记在该群体中并没有显示与穗发芽相关。本研究结果为定位控制该群体中穗发芽抗性的QTL位点提供了重要信息。

Pre-harvest sprouting in wheat significantly reduces end-use quality, seed value and yield. In this study, recombinant inbred lines （RILs） populations （Wanxian Baimaizi/Jing411） were used to detect validation of molecular marker mapped on the 3rd （XBarc321, XBarc310 and XBarc57） and 4th chromosomes （Xbarc170, Xgwm397 and Xgwm269） closely linked to pre-harvest sprouting resistance gene reported in previous study. The results indicated that molecular marker （XBarc321, XBarc310 and XBarc57） located on the 3rd chromosomes were more effective than molecular marker located on the 4th chromosomes. These results provide important information for mapping QTL loci associated with pre-harvest sprouting resistance in Wanxian Baimaizi/Jing411 populations.