利用新型分子标记EST-SSR鉴定湖北省内的主栽黑杨品种

Identification of Populus Varieties from Hubei Province by EST-SSR Marker

EST-SSR标记是基于表达序列标签(expressed sequence tags,EST)数据信息,并结合SSR标记特点开发出的一种新型分子标记。本文利用12对EST-SSR标记对湖北省内目前主要栽培的8个黑杨品种进行分子鉴定,同时对不同EST-SSR位点进行相关遗传参数分析。品种鉴定结果表明,仅需4对EST-SSR引物即可将8个亲缘关系较近的黑杨品种完全区别开来。遗传多样性分析显示,多态位点百分比为58.3%,平均有效等位基因数为2.09个,平均Shannon信息指数0.61,平均杂合度为0.35。这些结果表明,杨树EST-SSR标记完全可以应用于品种鉴定及群体遗传多样性分析。

The increasing availability of expressed sequence tags （EST） in public databases provides a valuable resource for development of new EST-SSR molecular markers. In this study, the 8 Populus varieties from Hubei province were identified by EST-SSR markers, and the genetic parameters on different loci were analyzed. The re- sult about variety identification showed that only 4 pairs of EST-SSR primers could totally differentiate 8 Populus varieties, and the genetic diversity analysis showed that polymorphic loci percentage was 58.3%, average effective number of alleles was 2.09, average Shannon＇s information index was 0.61, and average expected heterozygosity was 0.35. All these results indicated that EST-SSR markers from Populus can be used for the analysis of variety identification and population genetic diversity.