帕金森病患者体内氧化压力的定量分析

Estimation of oxidative stress in patients with Parkinson＇ s disease

目的测定帕金森病（PD）患者尿中8-异前列腺素F2α（8-iso-PGF2α）和血中低密度脂蛋白体外氧化延迟时间的变化。方法运用病例一对照观察，根据Calne标准选取31例男性PD患者、同时选取31例年龄、影响因素等相当的健康男性为对照。通过铜氧化共轭双烯法在波长234nm测定血中低密度脂蛋白体外氧化延迟时间以及酶联免疫法测定尿中8-iso-PGF2α浓度。结果PD患者组尿中8-iso-PGF2α含量比对照组明显增高[（81．1±1．6）ng／mmol肌酐vs（46．9±1．1）ng/mmol肌酐]，差异有统计学意义（P〈0．05）；而血中低密度脂蛋白体外氧化延迟时间明显减低[（63．5±6．0）min vs（84．4±8．8）min]，差异有统计学意义（P〈0．05）。结论增高的氧化压力与降低的抗氧化能力在PD的病理过程中具有一定的作用，提示有效的抗氧化治疗可能对控制疾病的发展具有一定的意义。

Objective To measure the changes in urinary 8-iso-prostaglandin-F2α（8-iso-PGF2α） and oxidized low-density lipoprotein （ox-LDL） lag time in patients with Parkinson＇s disease （PD）. Methods A case-control study was performed involving 31 male patients with PD （mean age of 59.7 years） diagnosed according to the Calne criteria and 31 age-matched healthy male subjects with comparable status of smoking and life style. For each subject, urinary 8-iso-PGF2α was measured quantitatively using enzyme-linked immunosorbent assay, and LDL oxidizability was measured by determining LDL oxidation lag time in conjugated diene product at 234 nm using Cu-stimulated oxidation. Results Compared to the levels in the control subjects, the PD patients showed significantly increased urinary 8-iso-PGF2α （81.1±1.6 vs 46.9±1.1 ng/mmol creatinine, P〈0.05） and significantly reduced LDL oxidation lag time （63.5 ±6.0 vs 84.4±8.8 min, P〈0.05）. Conclusion Increased 8-iso-PGF2α and decreased anti-oxidant ability are implicated in the pathogenesis of PD, suggesting the value of appropriate antioxidant therapy in controlling the progression of PD.