重组Sj-Ts4样蛋白在日本血吸虫病免疫诊断中的初步应用

Preliminary application of recombinant protein Sj-Ts4 in immunodiagnosis of Schistosomiasis japonica

目的评价重组sj—Ts4样蛋白对日本血吸虫病的诊断价值。方法取血吸虫病患者（急性、慢性、晚期）血清74份、华支睾吸虫病患者血清24份、钩虫病患者血清8份和健康人血清30份，利用纯化、重组rSi—Ts4样蛋白和日本血吸虫成虫抗原（SjAWA），采用EUSA法分别检测血清中抗rSi—Ts4样蛋白特异性抗体和SjAWA抗体，比较两种检测方法的敏感性和特异性。结果rSj—Ts4-ELISA和SjAWA—ELISA法检测急性、慢性和晚期血吸虫病患者血清的阳性率分别为97．1％（33／34）、100．0％（16／16）、87．5％（21／24）和100．0％（34／34）、100．O％（16／16）、75．0％（18／24），经校正x2检验，两种检查方法比较差异无统计学意义（X2=1．23，P〉0．05）；健康人血清两种检测方法的假阳性率分别为6．7％（2／30）和3．3％（1／30），经校正x。检验，差异无统计学意义（x2=0．35，P〉0．05）；华支睾吸虫患者和钩虫病患者血清阳性率分别为12．5％（3／24）、20．8％（5／24）和12．5％（1／8）、37．5％（3／8），经校正x。检验，两种检查方法比较差异无统计学意义（x2值分别为0．60、1．33，P〉0．05）。结论rSj—Ts4样蛋白作为抗原，对免疫检测日本血吸虫病患者血清中的抗体具有较好的敏感性和特异性．对日本血吸虫病患者的诊断有较大的应用价值。

Objective To evaluate the diagnostic value of the recombinant protein Sj-Ts4 in immunodiagnosis of Schistosomiasis japonica. Methods Seventy-four blood samples of schistosomiasis japonica patients （acute, chronic and advanced） were used for evaluating the sensitivity. Blood samples from 24 Clonorchiasis patients, 8 patients with hookworm infections and 30 normal persons from the areas without Schistosomiasis were used for evaluating the specificity. The rSj-Ts4-ELISA and SjAWA-ELISA were subjected to the serological diagnosis in these patients. Results The positivity rates were 97.1%（33/34）, 100.0%（16/16）,87.5%（21/24） in rSj-Ts4-ELISA and 100%（34/34）, 100.0%（16/16） ,75.0%（18/24） in SjAWA-ELISA in acute, chronic and advanced Schistosomiasis, respectively. Statistical analysis revealed no significant difference in sensitivity （X2 = 1.23, P 〉 0.05） between both recombinant and crude antigens. The false positive reaction was found to be 6.7% （2/30） in rSj-Ts4-ELISA and 3.3% （1/30） in SjAWA-ELISA when detected in 30 cases of normal control sera, but no statistically significant difference was noted（x2 = 0.35,P 〉 0.05）. Twelve point five percent（3/24）,20.8%（5/24） and 12.5%（1/8）,37.5% （3/8）, of cross-reactions were observed between rSj-Ts4-ELISA and SjAWA-ELISA for detecting the sera of patients with clonorchiasis and hookworms. There was no significant difference of cross-reaction in two parasitic infections （X2 = 0.60,1.33, P 〉 0.05） with the two tests. Conclusions The rSj-Ts4 antigen shows higher sensitivity and specificity for the diagnosis of Schistosomiasis japonica, which is helpful in the serological diagnosis of Schistosomiasis japonica in endemic areas.