小鼠FAAP蛋白对细胞黏附的影响

FAAP is Involved in Cell Attachment

黏着斑相关蛋白(focal adhesion associated protein,FAAP)由小鼠D10Wsu52e基因编码,在进化上十分保守,但该蛋白质的生物学功能并不清楚.为此,首先通过细胞组分分离的方法研究了FAAP蛋白分布的细胞组分,结果表明FAAP主要存在于细胞质和细胞膜中.细胞蛋白表达量分析表明,细胞中FAAP与黏连蛋白受体(LR)表达量呈现正相关性.同时,细胞黏附实验表明,FAAP与LR对细胞黏附影响类似,也能够抑制细胞的黏附.这些实验结果为深入研究FAAP蛋白功能提供了依据.

FAAP （focal adhesion associated protein）, encoded by murine DlOWsu52e gene, is a member of function-unknown protein family, UPF0027. These protein family homologues are conserved and ubiquitously expressed in various species, organs and cells. But the functions are quite unclear yet. Here, FAAP were demonstrated to deposited in the cytoplasmic fractions and the plasma membrane fractions by ultra-speed centrifugation. The expression levels of FAAP are significantly correlative with 67 ku Laminin receptor （LR） in HeLa cells. Moreover, FAAP, similar with LR, could effectively restrain the cultured cells attachment. Together, these results suggest the effects of FAAP on cell attachment.