提前光照对氧诱导视网膜病小鼠模型的视网膜血管发育的影响

The effects of premature light exposure on the development of new retinal vessels in a mouse model of oxygen-induced retinopathy

目的探讨提前光照对氧诱导视网膜病小鼠模型的视网膜新生血管发育的影响。方法48只健康C57BL/6J小鼠,随机取12只作为正常对照组,其余随机分为高氧模型组、提前光照组和高氧联合提前光照组,每组各12只小鼠。高氧模型组:将出生后第7天(P7)的小鼠置于含75%高浓度氧的氧箱内,5d后取出,再置于正常空气环境中饲养;提前光照组:取出生后第4天(P4)未开睑新生小鼠,手术提前开启右眼睑裂,让右眼提早接受环境光及视觉刺激,左眼为自身对照;高氧联合提前光照组:取P4小鼠提前开启右眼睑裂,在P7时放入高浓度氧箱内饲养,5d后取出。各组均于小鼠生后第27天(P27)取材,应用HE染色、视网膜铺片ADP酶染色及视网膜新生血管计数等方法观察小鼠视网膜新生血管的生长情况。结果在P27时,正常对照组和单纯提前光照组小鼠视网膜均无显著新生血管生长;高氧模型组内皮细胞计数较正常对照组及单纯提前光照组明显增加,有显著统计学意义(P<0.01),提示该组存在显著视网膜新生血管生长;在高氧联合提前光照组,双眼新生血管内皮细胞计数均较正常对照组明显增加(P<0.05),但该组的提前光照眼新生血管内皮细胞计数较单纯高氧作用眼有所减少(P<0.01)。结论对于小鼠,高氧可诱导视网膜新生血管的生成,提前光照可削弱高氧所致的小鼠视网膜新生血管的增殖。

Objective To investigate the effects of premature light exposure on retinopathy which was induced by oxygen in a mouse model. Methods Forty-eight healthy C57BL/6J mice were randomly divided into 4 groups： 12 mice in the control group, 12 mice were raised in a box with 75% oxygen from 1＇7 （postnatal day 7） to P12, the right eyelids of another 12 mice were surgically opened on P4 to be exposed to light prematurely, and the eyelids of the last 12 mice were opened on P4 and then raised in a box with 75% oxygen from 1~7 to P12. The eyeballs of all of the mice were removed on P27. The development of the retinal vessels was observed by ADPase histochemisty and the proliferative neovaseular response was quantified by counting the nuclei of new vessels extending from the retina into the vitreous in serial sagittal cross sections. Results New retinal vessels could not be observed in the control group and in the mice with prematurely opened eyelids, but could he clearly observed in the oxygen-induced retinopathy group. There were fewer nuclei in new vessels extending from the retina into the vitreous in mice raised in high-concentration oxygen with prematurely opened eyelids than in those without premature lid- opening. Conclusion The retina of mice raised in high-concentra- tion oxygen engendered neovaseularization. Premature light exposure can weaken the effect of a high concentration of oxygen.