尿苷肽类抗生素sansanmycin D的分离、纯化和结构鉴定

Isolation,purification and structure determination of uridylpeptide antibiotic sansanmycin D with activity against Pseudomonas aeruginosa

目的从尿苷肽类抗生素sansanmycins的产生菌Streptomyces sp.SS的发酵液中分离、提取和纯化小组分,并进行结构鉴定。方法以铜绿假单胞菌为检定菌,利用大孔树脂吸附、反相制备HPLC等方法进行分离纯化。用光谱分析,进行结构解析。结果分离到一个尿苷肽类化合物sansanmycin D。结论Sansanmycin D与文献报道的mureidomycin A结构一致。

Objective To isolate and purify the minor components from the fermentation broth of Strepto-myces sp. SS producing sansanmycins and determine their structures. Method Antibiotic activity was determined by a paper-disc agar diffusion assay using Pseudomonas aeruginosa. The active antibiotics were isolated and purified by means of various chromatographies, such as macroporous adsorption resins, reversed-phase HPLC, etc. Their struc-tures were elucidated by spectroscopic analyses. Result A uridylpeptide antibiotic sansanmycin D was obtained. Conclusion Sansanmycin D was designated as mureidomycin A.