bFGF特异性结合噬菌体的活性研究

Activity Study of Specific bFGF-Binding Phage

研究从噬菌体随机7肽库中筛选的bFGF特异性结合噬菌体的活性。采用ELISA检测3轮淘选后得到的阳性噬菌体克隆对bFGF的结合曲线。通过竞争抑制实验,分析bFGF结合噬菌体与bFGF的竞争结合活性。采用MTT法检测bFGF结合噬菌体对由bFGF诱导的Balb/c3T3细胞增殖的影响。从噬菌体肽库中淘选获得的阳性噬菌体克隆能够特异地与bFGF结合,并呈剂量依赖性,其与固相bFGF的结合能被游离的bFGF竞争抑制,并可抑制由bFGF诱导的Balb/c3T3细胞增殖。bFGF特异性噬菌体可拮抗bFGF的活性,为开发针对bFGF的抗肿瘤短肽类新药提供基础。

To study the activity of a specific bFGF-binding phage selected from a phage display heptapeptide library. EL1SA was applied to assess the bFGF-binding curve of the positive phage clone obtained from 3 rounds screening. The competitive binding activity of the bFGF- binding phage was examined by competitive inhibition assay. The effect of the bFGF-binding phage on the proliferation of Balb/c 3T3 cells induced by bFGF was investigated by M3T method. The positive phage selected from the phage library specifically band to bFGF in a dose-dependent manner, and its binding with the immobilized bFGF was competitively inhibited by free bFGF. The isolated phage could also inhibit the proliferation of Balb/c 3T3 cells induced by bFGF. The bFGF-binding phage could inhibit bFGF activity, which would offer the basis for further developing the anti-tumor short peptide targeting bFGF.