五种SPR传感芯片的再生制备及其应用

Methods for Regeneration of Five Kinds of SPR Sensor Chips and Their Applications

基于表面等离子体共振技术(surface plasmon resonance,SPR)的生物传感器,能够实时监测生物分子间的相互作用,且无需标记,已被广泛应用于蛋白质组学、药物研发、临床诊断、食品安全和环境监测等领域,并且显示出广阔的应用前景。传感芯片是Biacore系列仪器的核心部件,目前芯片只能从Biacore公司购买,价格昂贵,导致很多仪器利用率低下,资源处于闲置状态。阐述了用于Biacore系列仪器的五种传感芯片(J1,C1,CM5,SA和NTA芯片)的再生制备方法,并列举了应用实例,制备方法操作简单,成本低廉。通过多年的改进与优化,制备的芯片能够达到Biacore芯片同等品质。此方法的推广,将有助于推动表面等离子共振技术在各个领域的广泛应用。

The biosensor based on the principle of surface plasmon resonance （ SPR）, is a surface sensitive optical device for monitoring biomolecular interactions at the sensor surface in real time without any labeling. It is being used in a wide variety of areas such as proteomics, drug discovery, clinical diagnosis, food analysis, environmental monitoring and so on. The sensor chi Pis the core of the Biacore instrument. Since sensor chips can now only be purchased from Biacore AB （Uppsala, Sweden ）, which makes them expensive, many instruments are either used at a low rate or sleeping. Simple and cost-effective methods for the regeneration of five kinds of sensor chips for the Biacore instrument, which include the J1, C1, CMS, SA and NTA sensor chip, were described. And several applications of these regenerated sensor chips were listed. Through the improvement and optimization of these methods in the last four years, the regenerated sensor chi Pcan achieve the same quality as the Biacore sensor chip. The wide use of these regeneration methods will be highly beneficial to the application and development of SPR technology in a wider variety of areas.