摘要
NASBA(nucleic acid sequence—based amplification)即依赖核酸序列的扩增技术,是一种扩增RNA的新技术,是由一对引物介导的、连续均一的、体外特异核苷酸序列等温扩增的酶促反应过程。反应在42%进行,可以在2h左右将模板RNA扩增约10卧佗倍,不需特殊的仪器。NASBA已经广泛应用于细菌、病毒等多种病原微生物的检测,就NASBA的技术原理及其在病毒检测中的应用作一综述。
Nucleic acid sequence-based amplification (NASBA) is a sensitive, isothermal, transcription- based amplification system specifically designed for the detection of RNA targets, which could amplify templete RNA in 2h under isothermal condition at about 42~C and without any special equipment. NASBA is now widely applicated in diagnosis of many pathogenic microorganism. It is mainly about principles and applications of NASBA in viral diagnosis.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2009年第1期80-85,共6页
China Biotechnology
基金
国家“十一五”科技支撑计划资助项目(2006BAD06A14,2006BAD06A17)