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NASBA(依赖核酸序列的扩增)技术及其在病毒检测中的应用 被引量：23

Nucleic Acid Sequence-Based Amplification and Its Applications in Viral Diagnosis
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摘要 NASBA（nucleic acid sequence—based amplification）即依赖核酸序列的扩增技术，是一种扩增RNA的新技术，是由一对引物介导的、连续均一的、体外特异核苷酸序列等温扩增的酶促反应过程。反应在42％进行，可以在2h左右将模板RNA扩增约10卧佗倍，不需特殊的仪器。NASBA已经广泛应用于细菌、病毒等多种病原微生物的检测，就NASBA的技术原理及其在病毒检测中的应用作一综述。 Nucleic acid sequence-based amplification （NASBA） is a sensitive, isothermal, transcription- based amplification system specifically designed for the detection of RNA targets, which could amplify templete RNA in 2h under isothermal condition at about 42~C and without any special equipment. NASBA is now widely applicated in diagnosis of many pathogenic microorganism. It is mainly about principles and applications of NASBA in viral diagnosis.
作者 高闪电 常惠芸 丛国正 独军政 邵军军 林彤
机构地区 中国农业科学院兰州兽医研究所国家VI蹄疫参考实验室家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室
出处 《中国生物工程杂志》 CAS CSCD 北大核心 2009年第1期80-85,共6页 China Biotechnology
基金 国家“十一五”科技支撑计划资助项目(2006BAD06A14,2006BAD06A17)
关键词 依赖核酸序列的扩增(NASBA) 恒温扩增 病毒检测 diagnosis Nucleic acid sequence-based amplification NASBA Isothermal amplification Viral
分类号 Q52 [生物学—生物化学]
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参考文献27

  • 1Compton J. Nucleic acid sequence-based amplification. Nature, 1991, 350(7): 91-92
  • 2Rodrignez-Lazaro D, D'Agostino M, Pla M, et al. Construction strategy for an internal amplification control for real-time diagnostic assays using nucleic acid sequence-based amplification: development and clinical application. J Clin Microbiol. 2004, 42(12) :5832-5836
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  • 4Lau L T, Scott M R, Donald P K, Detection of foot-and-mouth disease virus by nucleic acid sequence-based amplification (NASBA). Vet Micrubiol, 2008, 126(1 -3) : 101-110
  • 5Weusten J J A M, Carpay W M, Tom A M,et al. Principles of quantitation of viral loads using nucleic acid sequence-based amplification in combination with homogenous detection using molecular beacons. Nucl Acid Res, 2002, 30, e26
  • 6Jean J, Blais B, Darveau A, et al. Rapid detection of human rotavirus using colorimetric nucleic acid sequence-based amplification (NASBA) -enzyme-linked immunosorbent assay in sewage treatment effluent. FEMS Microbiol Lett, 2002, 210 (1) : 143 -147
  • 7Matthew C S, George S, Stan I, et al. An integrated portable hand-held analyser for real-time isothermal nucleic acid amplification. Analytica Chimica Acta, 2007, 598 (2) : 286-294
  • 8Yao J, Liu Z, Lung-Sang K, et al. Quantitative detection of HIV-I RNA using NucliSens EasyQ HIV-1 assay. J Virol Methods, 2005, 129(1 ): 40-46
  • 9Joseph W R, Roxanne N S, Eric D, et al. Quantitative evaluation of simian immunodeficiency virus infection using NASBA technology. J Virol Methods, 2000, 86( 1 ) : 61-70
  • 10Jordan H L, Scappino L A feline immunodeficiency sequence based Methods, 2002, M amplification oscardini M, et al. Detection of RNA by two nucleic acid (NASBA) formats. J Virol 103(1): 1-13

二级参考文献62

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中国生物工程杂志
2009年 第1期

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