生物传感器检测纤维素酶活性及基因表达

Research Progress on Determination of Cellulase Activity and Gene Expression by Biosensor

纤维素酶是一类具有多组分的复杂酶系。纤维素酶及其水解木质纤维原料的作用机制、木质纤维原料酶水解动力学等领域的研究仍需要更深层次的分析探讨,从而完善对纤维素酶的研究与认识。随着生物化学、分子生物学技术以及基因工程等多门交叉学科的快速发展,如何进一步阐明纤维素酶的结构与功能,明确纤维素酶的基因表达与调控的关系,并由此拓展更多更好的研究方法、手段,在纤维素酶的研究方面将具有重要意义。通过协同催化作用方式和氨基酸序列的相似性介绍纤维素酶酶系的组成,概述纤维素酶各酶组分的传统检测方法,并侧重阐述各种传感器应用于检测纤维素酶活性及基因表达的进展。

Cellulase system contains a series of complex components. There are still some problems remained unclear in cellulase and its mechanism of hydrolyzing lignocellulosic materials and its hydrolysis kinetics, so profound study is needed. The rapid development of many kinds of new interdiscipline such as biochemistry, molecular biology and gene engineering, has further clarified the structure and function of cellulase, and the relationshi Pof its gene expression and regulation, and furthermore resulted in derivative study methods about cellulase in more aspects. Cellulase system components according to synergistic catalytic mode and the sequence of the homology amino acids similarity, summarizes traditional detection methods of enzyme components, and emphasizes on research progress of various biosensors applied in detection of cellulase activity and gene expression was introduced.