白介素10抑制大鼠脑缺血再灌注E、L-选择素表达的研究

Inhibiting effects of interleuin-10 on expression of E-selectin and L-selectin in cerebral ischemiareperfusion： experiment with rats

目的探讨白介素10（IL-10）对大鼠脑缺血再灌注后E-选择素及L-选择素表达的抑制作用。方法72只成年雄性sD大鼠随机均分为假手术组，脑缺血／再灌注组，溶剂对照组和IL-10干预组。IL—10干预组经侧脑室注射IL-10（1μg溶解于5μl5mol／LNaP），对照组经侧脑室注射溶剂（5μl5mol／LNaP）。免疫组织化学法、RT—PCR法和Western印迹法检测E-选择素及L-选择素的表达变化。结果脑缺血再灌注组E-选择素及L-选择素表达明显高于假手术组（P〈0．05）。IL-10干预组E-选择素及L-选择素阳性血管（18．8±1．9，15．8±2．4）明显少于溶剂对照组（24．7±2．4，20．9±3．3）（P〈0．05）。IL-10干预组E-选择素及L-选择素基因表达（0．431±0．029，0．318±0．048）明显低于溶剂对照组（0．497±0．019，0．433±0．087）（P〈0．05）。IL-10干预组E-选择素及L-选择素蛋白表达（0．349±0．037，0．296±0．035）明显低于溶剂对照组（0．421±0．043，0．348±0．044）（P〈0．05）。结论IL-10可抑制大鼠脑缺血再灌注后E-选择素及L-选择素的表达。

Objective To investigate the inhibiting effects of interleukin-10 （IL-10） on the expression of E-selectin and L-selectin in cerebral ischemia-reperfusions. Methods Seventy-two adult male Sprague-Dawley rats were randomly divided into 4 equal groups, cerebral ischemia-reperfusion （I/R） group undergoing middle cerebral artery occlusion with Longa＇s thread method, IL-10 group undergoing lateral ventricle injection of IL-10 after the establishment of I/R model, Vehicle group undergoing lateral ventricle injection of normal saline after the establishment of I/R model, and sham operation （Sham） group. Twenty- four hours later the rats were killed with their brains taken out. Immunohistochemistry, RT-PCR and Western blotting were used to detect the mRNA and protein expression of E-selectin and L-selectin. Results The E-selectin and L-selectin expression levels of the I/R group were significantly up-regulated compared with the Sham group （ both P 〈 0.05）. The numbers of E-selectin and L-selectin positive vessels of the IL- 10 group were 18.8 ± 1.9/10 HP fields and 15. 8 ± 2.4/10 HP fields respectively, both significantly less than those of the vehicle group （24.7 ± 2.4/10 HP fields and 20.9 ± 3.3/10 HP fields respectively, both P 〈0.05）. The E-selectin and L-selectin gene mRNA expression levels of the IL-10 group were （0. 431 ±0. 029） and （0. 318 ± 0. 048 ） respectively, both significantly lower than those of the Vehicle group [ （0.497 ±0.019） and （0. 433 ± 0. 087） respectively, both P 〈 0.05]. The E-selectin and L-selectin protein expression levels of the IL-10 group were （0.349 ± 0.037） and （0. 296 ± 0.035） respectively, both significantly lower t6han those of the Vehicle group [ （0. 421 ±0. 043, ） and （0. 348 ±0. 044） respectively, both P 〈 0.05 ]. Conclusions IL-10 suppresses the expression of E-selectin and L-selectin in cerebral ischemia- reperfusion.