期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

CHO细胞中稳定表达人整合素β3对登革2型病毒感染的影响 被引量：1

Effect of recombinant β_3 integrin on Dengue virus serotype 2 infection in CHO cells
下载PDF
导出
摘要 目的检测CHO细胞中稳定表达人整合素β3对登革2型病毒(Dengue virus serotype 2,DV2)感染的影响。方法将表达人整合素β3的质粒转染CHO细胞,通过G418筛选、流式细胞学检测和间接免疫荧光实验,建立稳定表达整合素β3的CHO细胞株;利用该细胞株进行感染实验,检测人整合素β3稳定表达对DV2易感性的影响。结果共获得了C9、C10、F8和H6 4个高表达整合素β3的细胞克隆,其中克隆F8中整合素β3的表达水平最高,是对照组的1.89倍。感染实验结果显示,进入整合素β3高表达细胞株的病毒数量均有所增高,增高的比例与整合素β3的表达增高趋势基本一致,进入F8细胞株的病毒量最高,是对照细胞的2.63倍,说明整合素β3有助于DV2进入细胞。结论在CHO细胞中外源性表达整合素β3可以提高DV2的感染率。 Objective To study the effect of recombinant β3 integrin on Dengue virus serotype 2 （DV2） infection of CHO cells. Methods Expression plasmid coding β3 integrin was transfeeted into CHO cells using the lipofectamine reagent. Stable CHO cell lines expressing human β3 integrin were selected in G418 and valida- ted by flow eytometry and indirect immunofluorence. The stable cell lines were used to determine whether β3 integrin expression facilitates DV2 infection. Results The stably transfeeted CHO variant cell lines C9, C10, F8 and H6 expressing human 153 integrin were selected. The level of β3 integrin expression in F8 was the highest, which was 1.89-fold of parental CHO cells. All the variant cell lines were more susceptible to DV2 infection, and F8 cell line was especially 2.63 times of parental CHO cells. Conclusion Recombinant β3 integrin promotes DV2 infection of CHO cells.
作者 张俊磊 高娜 陈炜 陈宗涛 徐小峰 安静
机构地区 第三军医大学基础医学部微生物学教研室 第三军医大学基础医学部组织学与胚胎学教研室 首都医科大学微生物学教研室
出处 《第三军医大学学报》 CAS CSCD 北大核心 2009年第2期98-100,共3页 Journal of Third Military Medical University
基金 国家自然科学基金(30640065,30671853) 北京市自然科学基金(5082004) 第三军医大学科研创新基金(2007XG15)~~
关键词 登革病毒 整合素Β3 CHO细胞 Dengue virus β3 integrin CHO cells
分类号 R373.33 [医药卫生—病原生物学] R394.33 [医药卫生—医学遗传学]
  • 相关文献

参考文献6

  • 1Zhang J L, Wang J L, Gao N, et al. Up-regulated expression of beta3 integrin induced by dengue virus serotype 2 infection associated with vires entry into human dermal mierovascular endothelial cells[J]. Biochem Biophys Res Commun, 2007, 356(3): 763 -768.
  • 2张俊磊,蹇锐,万颖杰,彭涛,安静,张复春,唐小平.广州2002年登革病毒流行株的分离鉴定及进化分析[J].第三军医大学学报,2005,27(4):282-286. 被引量：5
  • 3Peyrefitte C N, Pastorino B, Grau G E, et al. Dengue virus infection of human microvascular endothelial cells from different vascular beds promotes both common and specific functional changes[ J]. J Med Virol, 2006, 78(2) : 229 -242.
  • 4Guerrero C A, Mendez E, Zarate S, et al. Integrin alphavbeta3 mediates rotavirus cell entry [ J]. Proc Natl Acad Sci U S A, 2000, 97 (26) : 14644 - 14649.
  • 5Chu J J, Ng M L. Interaction of West Nile virus with alpha v beta 3 integrin mediates virus entry into cells [ J ]. J Biol Chem, 2004, 279 (52) : 54533 -54541.
  • 6Akula S M, Pramod N P, Wang F Z, et al. Integrin aipha3betal (CD 49c/29) is a cellular receptor for Kaposi' s sarcoma-associated herpesvires (KSHV/HHV-8) entry into the target ceils[ J]. Cell, 2002, 108 (8) : 407 -419.

二级参考文献14

  • 1秦鄂德.登革病毒基因组逆转录及其cDNA扩增的研究[J].中华微生物和免疫学杂志,1991,11(5):324-324.
  • 2Leitmeyer K C, Vaughn D W, Watts D M, et al. Dengue virus structural differences that correlate with pathogenesis[J]. J Virol, 1999, 73(6): 4738-4747.
  • 3Aviles G, Rowe J, Meissner J, et al. Phylogenetic relationships of dengue-1 viruses from Argentina and Paraguay[J]. Arch Virol, 2002, 147(11): 2075-2087.
  • 4Chu M C, O'Rourke E J, Trent D W. Genetic relatedness among structural protein genes of dengue 1 virus strains[J]. J Gen Virol, 1989, 70(pt7): 1701-1712.
  • 5Rico Hesse R. Molecular evolution and distribution of dengue viruses type 1 and 2 in nature[J]. Virology, 1990, 174(2): 479-493.
  • 6Goncalvez A P, Escalante A A, Pujol F H, et al. Diversity and evolution of the envelope gene of dengue virus type 1[J]. Virology, 2002, 303(1): 110-119.
  • 7Chungue E, Cassar O, Drouet M T, et al. Molecular epidemiology of dengue-1 and dengue-4 viruses[J]. J Gen Virol, 1995, 76(pt7): 1877-1884.
  • 8Yamada K, Takasaki T, Nawa M, et al. Virus isolation as one of the diagnostic methods for dengue virus infection[J]. J Clin Virol, 2002, 24(3): 203-209.
  • 9Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees[J]. Mol Biol Evol, 1987, 4(4): 406-425.
  • 10Page R D. Treeview: an application to display phylogenetic trees on personal computers[J]. Comput App Biosci, 1996, 12(4): 357-358.

共引文献4

同被引文献17

  • 1Lin CF,Lei HY,Shiau AL,et al.Endothelial cell apoptosis induced by antibodies against dengue virus nonstructural protein 1 via pinduction of nitric oxide[J].J Immunol,2002,169(2):657-664.
  • 2Murgue B,Cassar 0,Deparis X.Plasma concentrations of 9VCAM-1 and severity of dengue infections[J].J Med Virol,2001,65(1}97-104.
  • 3Borges E,Jan Y,Ruoslahti E.Platelet-derived growth factor receptor beta and vascular endothelial growth factor receptor 2 bind to the beta 3 integrin through its extracellular domain[J].J Biol Chem,20O0,275(51):39867-39873.
  • 4Gavrilovskaya IN,Peresleni T,Geimonen E,et al.Pathogenic hantaviruses selectively inhibit beta3 integrin directed endothelial cell migration[J].Arch Virol,2002,147(10):1913-1931.
  • 5Zhang JL,Wang JL N,et al.Up-regulated expression of beta3 integrin induced by dengue virus serotype 2 infection associated with virus entry into human dermal microvagcular endothelial cells[J].Biochem Biophys Res Commun,2007,356(3):763-768.
  • 6Thepparit C,Smith DR.Serotype-specific entry of dengue virus into liver cells:identification of the 37-kilodalton/67-kilodalton highaffinity laminin receptor as a dengue virus serotype 1 receptor[J].J Viral,2004,78(22):12647-12656.
  • 7Jindadamrongwech S,Thepparit C,Smith DR.Identification of GRP 78(BiP) as a liver cell expressed receptor element for dengue virus serotype 2[J].Arch Virol,2004,149(5):915-927.
  • 8Reyes-Del Valle 1,Chdvez-Salinas S,Medina F,et al.Heat shock protein 90 and heat shock protein 70 are components of dengue virus receptor complex in human cells[J].J Viral,2005,79(8):4557-4567.
  • 9Muitoz-Jordan JL,Sfinchez-Burgos GG,Laurent-Rolle M,et al.Inhibition of interferon signaling by dengue virus[J].Proc Natl Acad Sci U S A,2003,100(24):14333-14338.
  • 10Brandler S,Ruffie C,Najburg V,et al.Pediatric measles vaccine expressing a dengue tetravalent antigen elicits neutralizing antibodies against all four dengue viruses[J].Vaccine,2010,28(41):6730-6739.

引证文献1

第三军医大学学报
2009年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部