粘附因子CD44_S、CD44_(V3)、CD44_(V6)在乳腺癌中的表达及临床意义

Expression of CD44_S、CD44_(V3) and CD44_(V6) proteins in breast cancer and its relevance with clinical significance

目的:研究粘附因子CD44S、CD44V3、CD44V6蛋白在乳腺癌组织中的表达及其临床意义。方法:收集60例经根治性切除的乳腺癌标本,应用免疫组化S-P法检测CD44S、CD44V3、CD44V6蛋白的表达,并分析与临床病理因素之间的相关性。结果:CD44S、CD44V3、CD44V6在乳腺癌中的阳性率分别为78.3%、75.0%、78.3%。CD44S、CD44V6的表达程度与淋巴结转移、临床分期、肿块的大小密切相关(P<0.05)。CD44V3与乳腺癌的转移、临床分期无明显相关性。三者与患者的年龄均无明显相关性。结论:CD44S和CD44V6蛋白表达与乳腺癌转移、侵袭明显相关,而CD44V3则与之无明显相关性。

Objective：To evaluate the expression of CD44s and CDv3 ,CDv6 protein in breast cancer and its clinical significance. Methods：Sixty cases of formalin-fixed paraffin-embedded breast cancer were retrospectively analyzed using S-P immunohistochemical method with the monoclonal antibody CD44s,CD44v3 and CD44v6. The prognostic value of the CD44s ,CD44v3 and CD44v6 was assessed using nnivariate and multivariate survival analysis. Results：Positive rates of CD44s ,CD44v3 ,CD44v6 expression in breast cancer were 78. 3% , 75.0% and 78. 3%. There was significant correlation between CD44s ,CD44v6 immunoreactivity and tumor location, lymph node metastasis, and Dukes＇ stage （P 〈 0. 05）. CD44v3 expression will not be viewed as an independent prognostic indicator. Conclusion：CD44s and CD44v6 might play some important roles in metastasis of breast cancer, and CD44v3 expression might not be an useful independent prognostic marker of breast cancer cell.