木材锯末与谷壳烟雾致豚鼠肺部炎性反应和氧化-抗氧化失衡

Pulmonary inflammation and imbalance of oxidation/antioxidation caused by exposure to serrago and hull fumes in guinea pigs

目的探讨小材锯末和谷壳烟雾致豚鼠肺部的异常炎性反应及氧化损伤作用。方法20只白化豚鼠随机分为两组，每组10只。实验组每天暴露于木材锯末和谷壳燃烧所产生的烟雾巾两次，每次30min，共21d；对照组暴露于新鲜空气。3周后进行肺组织病理检测，并检测肺组织匀浆中的谷胱甘肽过氧化物酶（GSH—PX）、还原型谷胱甘肽（GSH）、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、总抗氧化能力及肺泡灌洗液（BALF）中的MDA、SOD、总抗氧化能力、白细胞介素8（IL-8）、IL-6、细胞问黏附分子1（ICAM-1）的活力或含量。结果与对照组豚鼠比较，实验组肺实质、小动脉及支气管炎性细胞浸润明显增多；肺泡面积比例及平均肺泡面积明显增大；单位面积肺泡数明显减少[（134．11±18．22）个／视野比（178．76±21．72）个／视野，P〈0．01]；肺小动脉及支气管平滑肌层显著增厚。肺组织匀浆中SOD、GSH—PX、GSH、CAT、总抗氧化能力的活力或含量降低[（225．55±12．52）U／mg比（296．57±13．46）U／mg，（23．60±1．85）mg／g比（34．38±1．85）mg／g，（119．67±9．61）mg／g比（134．73±8．07）mg／g，（7．10±0．21）U／mg比（9．20±0．24）U／mg，（0．89±0．10）U／mg比（1．27±0．15）U／mg，P均〈0．01．]；MDA的含量显著升高[（3．05±0．38）U／mg比（2．09±0．17）U／mg，P〈0．01]。BALF中SOD、总抗氧化能力的活力显著降低；MDA的含量显著升高；BALF中IL-6、IL-8、ICAM-1浓度增高。结论木材锯末与谷壳烟雾可导致豚鼠肺组织及气道氧化-抗氧化失衡，引起氧化损伤。

Objective To explore the abnormal inflammation and oxidative damage of lung tissues in guinea pigs exposed to serrago and bull fumes. Methods Twenty albino guinea pigs were divided randomly into two groups. The experiment group （n = 10） was exposed to sen＇ago and hull fumes for 30 minutes twice per day for 21 days and the control group （n = 10） exposed to fresh air. At the end of 3 weeks, animals were sacrificed and lung tissues were examined histopathologically. The activities of glutathione peroxidase （ GSHPX）, superoxide disumtase （SOD）, catalase （CAT） and the levels of reduced glutathione （GSH）, malondialdehyde （MDA） , total ability of antioxidant in lung tissue homogenate were measured. The activities of SOD and the levels of interleukin-8 （ IL-8 ） , IL-6,intercellular adhesion molecule-1 （ ICAM-1 ） , MDA and total ability of antioxidant in the bronchoalveolar lavage fluid （BALF） were measured. Results Compared to the control group, inflammatory cells infiltration in lung parenchyma, arteriole and bronchus iucreased obviously in experiment group. The alveolar area proportion and mean alveolar area were increased respectively, and the number of alveolar per square area was decreased significantly in experiment group （ 134.11± 18.22） per vision vs （ 178.76 ±21.72） per vision , P 〈 0.011. The thicknesses of pulmonary arterioles and brochial smooth muscle layer were significantly increased in the experiment group respectively. The levels of SOD, GSH-PX, GSH, CAT, total ability of antio xidant in lung tissue homogenate were lower in the experiment group than those in the control group [ （225.55± 12.52）U/mg vs（296.57 ±13.46）U/mg, （23.60 ± 1.85）mg/g vs（34.38 ±1.85）mg/g, （119.67 ±9.61） mg/g vs（134.73 ± 8.07）mg/g, （7. 10± 0.21 ） U/mg vs（9. 20 ±0. 24）U/rag, （0.89 ± 0.10） U/mg vs （1.27± 0.15 ）U/mg, all P 〈 0. 01 l, while MDA in lung tissue homogenate was significantly higher [ （ 3.05 ± 0.38 ） U/mg vs （ 2.09 ± 0. 17 ） U/rag, P 〈 0.01 ]. The SOD levels and total ability of antioxidant in BALF were lower in the experiment group than those in the control group,while MDA in BALF was significantly higher. The levels of IL-6, IL-8 and ICAM-1 in BALF were higher in the experiment group than those in the control group. Conclusion Serrago and hull fumes can cause the imbalance of oxidant/antioxidant and oxidative damage of lung tissue in the guinea pigs.