细胞因子信号转导抑制因子3在脓毒症小鼠肝脏和脾脏中的表达

Expression of suppressor of cytokine signaling 3 in liver and spleen of septic mice

目的探讨细胞因子信号转导抑制因子3（SOCS3）在脓毒症小鼠肝脏和脾脏中的表达情况及其可能的作用机制。方法采用盲肠结扎并穿刺术（CLP）制作小鼠脓毒症模型。检测肝脏和脾脏组织的SOCS3 mRNA及蛋白表达，采用RT—PCR检测组织中SOCS3 mRNA的相对含量，用免疫印迹方法测定组织中SOCS3相对蛋白含量。用SPSS统计软件对上述指标之间的变化关系进行分析。结果脓毒症手术后SOCS3在肝脏内基因和蛋白表达量有升高趋势，但与对照组比较差异无统计学意义（P〉0．05）。SOCS3在脾脏内的基因和蛋白表达在术后2h迅速升高，至12h达峰值。在肝脏和脾脏中SOCS3的基因表达和蛋白表达均呈正相关（r=0．353、0．731，P均〈0．05）。结论CLP导致的脓毒症可以诱导SOCS3在小鼠肝脏和脾脏中表达增多，提示SOCS3在脓毒症后的免疫变化中有重要作用。

Objective To investigate the expression change of suppressor of cytokine signaling 3 （SOCS3） in the liver and the spleen of septic mice, and to find out its possible mechanism in sepsis. Methods Cecal ligation and puncture （CLP） was adopted to make the sepsis model in mice. The liver and the spleen tissue were taken to detect the mRNA and protein expression of SOCS3. The level of SOCS3 mRNA expression was detected by RT-PCR, and the level of SOCS3 protein expression was examined by Western blot. SPSS statistics software was used to analysis the correlation between the expression of SOCS3 mRNA and protein. Results The gene and protein expression of SOCS3 only showed a trend of up-regulation in the liver after CLP, and there was no difference comparing with the control group （P 〉 0.05）. The expressions of SOCS3 gene and protein in the spleen were obviously higher than those in the control group at 2 h and reached the peak at 12 h after operation. Positive correlation was found between gene and protein expression in the liver and spleen（r = 0. 353, 0. 731, all P 〈 0.05）. Conclusions CLP-induced sepsis can up-regulate the expression of SOCS3 in mice, indicating that SOCS3may play an important role in the change of immune system in sepsis.