葡聚糖包被的纳米磁粒子制备及其在H-22细胞标记中的应用

PREPARATION OF SUPERMAGNETIC DEXTRAN-COATED IRON OXIDE NANOPARTICLES AND USING FOR H-22 CELLS MAGNETIC LABELING

利用化学共沉淀法制备近微米级葡聚糖T-40包被的超顺磁纳米氧化铁(SPIO)粒子。用X射线衍射(XRD)、透射电镜(TEM)、原子力显微镜(AFM)、红外光谱等手段对其性质进行表征。XRD确定所制备的粒子主要为Fe3O4晶体,具有超顺磁性且饱和磁化强度为64.396emu/g;TEM显示无机核心粒径约为20nm;被葡聚糖包被后,AFM显示粒子呈扁平长方体形貌,三维尺寸为(200～300)nm×(400～600)nm×(50～70)nm。在超声作用下,该类粒子能够对小鼠H-22肝癌细胞进行标记,说明所制备的磁性纳米粒子具有作细胞磁性标记物的特性,同时超声也可促进H-22细胞快速装载磁性微粒。

To improve a method of synthesizing dextran-coated superparamagnetic iron oxide （SPIO） nanoparticles and used as markers for H-22 tumor cells magnetic labeling in vitro. With the chemical precipitation method, the authors succeeded to get the SPIO particles. Under the transmission electron micrograph, the dextran-coated supermagnetic iron oxide nanoparticles were synthesised by the co-precipitation method. The size, shape and formation of the particles were investigated by atomic force microscope （AFM）, transmission electron microscopy （TEM） and x-ray diffraction analysis. While its magnetic character could be assessed by magnetic hysteresis loops and the presence of dextran in the magnetic-fluid had been confirmed by fourier transform infrared spectrometer （FTIR）. Cell loading of SPIO nanoparticles was facilitated by ultrasound. The core size of the samples is about 20 nm, The shape of the particle coated by dextran was cubic and the dimension size was （200-300）nm x （400-600）nm x （50 -70） nm, no magnetic hysteresis loop was observed and magnetic saturation density was 64.396 emu/g. At last, cells were labeled instantly by ultrasound in H-22 cells suspensions in vitro. Results indicated that the SPIOs prepared by chemical co-precipitation method could be used as markers for cellular magnetic labeling and ultrasound irradiation could be used to deliver iron oxide nanoparticles into H-22 cells.