胃癌中COX-2、PD-ECGF、VEGF的表达与血管生成的关系

The Relationship between the Expression of COX-2,PD-ECGF and VEGF in Human Gastric Carcinoma and Tumor Angiogenesis

目的研究探讨胃癌组织中COX-2、PD-ECGF、VEGF的表达与胃癌生物学行为及血管生成的关系。方法应用免疫组化ElivisionTM法检测胃癌、正常胃黏膜、肠化、不典型增生组织中COX-2、PD-ECGF、VEGF、CD34的表达并测定MVD,回顾分析胃癌病人的临床病理资料。结果胃癌组织中COX-2、PD-ECGF、VEGF的表达及MVD高于正常胃黏膜、肠化、不典型增生组,且与淋巴结转移、浸润程度密切有关。COX-2、PD-ECGF、VEGF表达阳性组的MVD高于各自阴性组。三者表达呈正相关关系,联合表达时MVD增高。结论COX-2、PD-ECGF、VEGF、血管生成参与了胃癌的发生、浸润与转移;COX-2、PD-ECGF、VEGF参与了胃癌的血管生成;表达存在协同作用;联合表达时血管生成的效应增强。三者作为肿瘤抗血管治疗的靶点具有一定的价值。

Objective To explore the expression of COX -2,PD -ECGF and VEGF in gastric carcinoma tissue,and analyze and identify the relationship between the expression and tumor angiogenesis together with biological behaviour in gastric carcinoma. Methods ElivisionTM immunohistochemical method was performed to detect the expression of COX -2, PD - ECGF, VEGF and CD34, and evaluate the value of MVD in surgically resected gastric carcinoma specimens and biopsy tissues under gastroscope,including normal gastric mucosa tissues, metaplasia tissues and gastric mucosal atypical hyperplasia tissues. Clinicopathologic data of patients were analyzed retrospective- ly. Results The positive expression of COX - 2, PD - ECGF,VEGF and MVD in gastric carcinoma were significantly higher than those in normal gastric mucosa, metaplasia or gastric mucosal atypical hyperplasia, and also their expression and MVD were closely related to lymph node metastasis and depth of invasion. The MVD in the groups of COX - 2, PD - ECGF, VEGF positive - expression were signifi- cantly higher than those in negative -expression groups respectively. There were positive correlations among the expression of COX -2 ,PD -ECGF and VEGF in gastric carcinoma. MVD increased significantly when COX -2, PD -ECGF and VEGF coexpressed. Conclusion The expression of COX - 2, PD - ECGF, VEGF and tumor angiogenesis participated in the genesis, invasion and metastasis of human gastric carcinoma. COX -2, PD - ECGF and VEGF participated in the tumor angiogenesis of gastric carcinoma. In the process of tumor angiogenesis and expression in gastric carcinoma, COX - 2, PD - ECGF and VEGF could strengthen the effectiveness each other. They could strengthen the effective Mess of tumor angiogenesis when they coexpressed. They could be selected as targets for tumor anti - angiogenesis treatment.