乌司他丁抑制肝切除术后高迁移率族蛋白1的释放及对肝功能的保护作用

High Mobility Group Box-1 Protein Inhibition and Liver Function Protection of Ulinastatin in Patients Underwent Hepatoectomy

目的观察乌司他丁(ulinastatin,UTI)对肝切除术后患者血浆高迁移率族蛋白1(Hiigh mobility group box-1 protein,HMGB1)水平的影响及其对肝功能的保护作用。方法121例患者随机分为:对照组(58例)和乌司他丁组(63例),分别于术前1天和肝切除术后1,3,5,7天采用酶联免疫吸附法检测血浆高迁移率族蛋白1活性,采用全自动生化分析仪检测谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(TBIL)水平。结果乌司他丁治疗组ALT、AST、HMGB1活性下降速度明显快于对照组(P<0.05);乌司他丁对TBIL的影响明显滞后于ALT和AST的变化,但是下降速度仍然快于对照组(P<0.05)。结论乌司他丁可能抑制HMGB1释放,对肝切除术患者肝功能具有保护作用。

Objective To investigate the effect of the ulinastatin on high mobility group box - 1 （HMGB1） protein and the liver function protection in patients undergoing hcpatoectorny. Methods 121 patients scheduled for bepatoectomy were randomly divided into two groups： control group （58cases） and ulinastatin group（63cases）. Plasma level of the HMGB1 protein activation was detected by enzyme -Linked Immunoadsorbent Assay（ELISA）. Alanine aminotransferase （ALT） , aspartate aminotransferase （AST） and total bilirubin （TBIL） were measured by automatic biochemistry analyzer at 1 day before and I, 3, 5, 7 days after the hepatoectomy respectively. Re- sults Compared to control group, the ALT,ALT and HMGB1 decreased more rapidly（P 〈 0. 05）. The influence on TBIL of ulinastatin was lagged of enzame change apparently, but the decreasing speed was still faster than that of control group （ P 〈 0. 05）. Conclusion Ulinastatin can decrease the plasma HMGB1 level and then protect the liver function of the patients accepting hepatoeetomy.