中药姜黄的两种提取物对肝癌细胞生长抑制的比较

Comparison of inhibitive effects of Curcumin and Calebin-A on human hepatoma cell line HepG2

目的:比较传统中药姜黄的两种提取物姜黄素(Curcumin)和Calebin-A对肝癌细胞(HepG2)的生长抑制作用.方法:应用MTT比色法检测姜黄素和Calebin-A对肝癌细胞体外生长的抑制作用;平板克隆检测两种提取物处理前后HepG2细胞的克隆形成能力;TUNEL法测定两种药物诱导肝癌细胞凋亡的作用.结果:姜黄素和Calebin-A对肝癌细胞的生长均有抑制作用,且随药物作用的浓度升高和时间延长,抑制效果增强.同一浓度下Calebin-A对细胞生长的抑制作用大于姜黄素,姜黄素和Calebin-A作用肝癌细胞48h后,半数细胞抑制作用所需浓度(IC50)分别约为25μmol/L和10μmol/L.相同浓度时(25μmol/L)两种提取物均可使肝癌细胞的克隆形成能力明显下降,且Calebin-A对细胞克隆形成能力影响更大.25μmol/L姜黄素及Calebin-A作用肝癌细胞48h后,Calebin-A诱导细胞发生凋亡的比例明显大于姜黄素.结论:姜黄素和Calebin-A对肝癌细胞的生长均有抑制作用,而且Calebin-A这种新的植物姜黄提取物对细胞的抑制作用更强.

AIM： To compare the inhibitive effects of Curcumin and Calebin-A on proliferation of human hepatoma cell line HepG2 cells. METHODS： MTF assay was used to evaluate the IC50 of Curcumin and Calebin-A on the cells. Colony formation assay was used to detect the ability of cell proliferation, when exposed to Curcumin and Calebin-A at 25 μmol/L. Cell apoptosis induced by two drugs was analyzed by TUNEL assay. RESULTS： Both Curcumin and Calebin-A inhibited the growth of HepG2 cells in a dose- and time-dependent manner. But Calebin- A showed higher ability in inhibiting the growth of HepG2 cells than Curcumin at the same concentration. After 48 h treatment, the IC50 of Curcumin and Calebin-A on HepG2 cells were about 25, and 10 μmol/L respectively. When the cells were exposed to 25 μmol/L Curcumin and Calebin-A, Calebin-A showed higher ability to inhibit the colony formation of HepG2 cells than Curcumin. Calebin-A induced the apoptosis of more HepG2 cells compared with Curcumin at the same concentration. CONCLUSION： Both Curcumin and Calebin-A could inhibit proliferation of HepG2 cells. But the new Curcuma longa constituent Calebin-A shows higher ability in inhibiting the growth of HepG2 cells than Curcumin.