抗心衰复方对豚鼠慢性心力衰竭的实验研究

Effect of a Traditional Chinese Medicine Compound on Chronic Heart Failure in Guinea-pigs

目的探讨抗心衰复方片(CTMC)对豚鼠慢性心力衰竭(CHF)心脏功能的影响。方法采用皮下反复注射异丙肾上腺素6周,制作豚鼠心肌缺血型CHF模型。观察CTMC对CHF豚鼠血流动力学、脏器/体质量比、病理改变和血清心肌肌钙蛋白-I(cTn-I)、肌酸激酶同工酶(CK-MB)含量的影响。结果CHF动物模型制作成功;CTMC可明显降低左室舒张压和左室舒张末压,中、大剂量组尤为显著;可显著提高左室收缩和舒张最大速率的绝对值,大剂量组更显著;明显减轻心肌细胞损伤和坏死;并可降低血清cTn-I、CK-MB含量,差异有统计学意义(P<0.01)。结论抗心衰复方片可改善CHF心脏的舒张功能和心肌细胞损伤,并呈现药物剂量依赖性。

Objective To investigate the effect of a traditional Chinese medicine compound （CTMC） on chronic heart failure （CHF） in guinea-pigs. Methods The CHF of guinea-pigs were induced by repeated injection of hyodemic isoproterenol. The hemodynamics, organ （heart, lung, liver and kidney）/body weight ratio, pathological changes, and serum cTn-I and CK-MB were measured to determine the effectiveness of the traditional Chinese medicine treatement. Results The LVDP and LVEDP were decreased and the absolute value of ＋-dp/dtmax and -dp/ dtmax were increased by the administration of 10 mg/kg, 20 mg/kg and 30 mg/kg of the compound tablets. The effect increased with doses. The traditional Chinese medicine also decreased the area of myocardial necrosis and the degree of injury to myocardiacyte. The intervention group had lower serum cTn-I and CK-MB levels than the controls. Conclusion The compound tablets can improve the left ventricular diastolic function of CHF and reduce the myocardial damage in a dose-dependent manner.