山核桃青果皮对几种人类致病细菌的抑制作用

Inhibition Effect of Green Walnut Peel on Several Human Pathogenic Bacteria

采用山核桃(Juglans mandshurica Maxim)青果皮作为实验材料,应用室内模拟实验、平板菌落计数法测定山核桃青果皮对空气中5种人类致病细菌的抑制效果,利用气质联用仪(GC/MS)测定山核桃青果皮中的挥发性成分。结果表明,山核桃青果皮对5种人类致病细菌有明显的抑制作用,且抑菌效果不同。抑制表皮葡萄球菌效果最好,抑菌率为83.3%;其次为金黄色葡萄球菌、猪霍乱沙门氏菌、肺炎球菌,抑菌率分别为75.4%、70.2%、62.6%;对痢疾志贺氏菌抑菌效果较差,为41.1%。通过对山核桃青果皮挥发物质的分析,共鉴定出24种挥发性成分。经查阅文献,初步确定胡椒烯、金合欢醇、氧化石竹烯、α-杜松醇为抑菌有效成分。

An experiment was conducted to study the bacteriostasis effect of green walnut peel on five kinds of human pathogenic bacteria in the air by plate count method through indoor simulation experiment. The volatile bactericidal components of green walnut peel were measured by GC/MS. Result indicates that green walnut peel has a significant effect on these five kinds of human pathogenic bacteria and the baeteriostasis effects are different. The bacteriostatie rates of green walnut peel to Staphylococcus epidermidis, Staphylococcus aureus, Salmonella choleraesuis, Diplococcus pneumoniae, and Shigella dysenteriae were 83.3% , 75.4% , 70.2% , 62.6% and 41.1% respectively. Twenty-four species of volatile bactericidal substances were detected by GC/MS. Copaene, farnesol, earyophyllcne oxide, and alpha-cadinol are preliminarily determined as active components with bacteriostasis effect by literature consulting.