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雌性鸽特异性序列的克隆测序

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摘要 试验根据原鸽雌性特异性序列AY944219设计引物,用PCR法分别扩增肉鸽和信鸽雌性特异性序列,并克隆测序。结果在肉鸽和信鸽分别克隆出长度为191bp的片段,且证实两个片段亦是雌性特有。把克隆测序获得的两段序列与GenBank上原鸽的序列(AY944219)进行两两比对,发现肉鸽与原鸽的同源性为98%,信鸽与原鸽的同源性为96%,而肉鸽与信鸽的同源性为97%。
出处 《国外畜牧学(猪与禽)》 2009年第1期71-73,共3页 Animal Science Abroad-Pigs and Poultry
基金 高产鸽选育及饲养技术的研究与应用 常州市科技局新农村建设项目
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