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抗小鹅瘟卵黄抗体脱脂方法研究 被引量:1

Study on Degreasing Methods in Egg Yolk Antibodies Against Gosling Plague
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摘要 通过对产蛋母鸡免疫接种小鹅瘟JS1株油乳剂灭活苗,从而获得含高效价小鹅瘟病毒抗体的鸡蛋。采用聚乙二醇6000和泊洛沙姆进行脱脂试验,并优化脱脂条件。结果表明,聚乙二醇6000和泊洛沙姆对鸡卵黄具有良好的脱脂作用,其中聚乙二醇6000价格低廉,更适合大规模工业化生产。 Eggs obtained from hens which had been immunized with gosling plague vaccine were used to prepare the yolk antibodies. The different concentrations of the PEG-6000 and pluronic were used to degrease the yolk. The results indicated that pluronic and PEG-6000 were all good for degreasing the yolk. PEG 6000 was more suitable for industrialized production of yolk antibodies because of its low price.
出处 《动物医学进展》 CSCD 北大核心 2009年第1期38-40,共3页 Progress In Veterinary Medicine
基金 江苏省三项工程(5410607)
关键词 卵黄抗体 泊洛沙姆F68 聚乙二醇6000 脱脂 egg yolk antibody pluronic F68 PEG-6000 degreasing
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  • 1胡晓苗,李春芬,汪丽,赵磊.高免卵黄抗体提纯及提取液冻干的研究[J].安徽农业科学,2006,34(10):2147-2147. 被引量:3
  • 2AKITA E M,NAKAI S. Comparison of four purification methods forthe production of immunoglobulins from eggs laid by hens immunizedwith an enterotoxigenic E. coli strain [ J ]. J Immunol Methods,1993,160(2) : 207 -214.
  • 3NTAKARUTIMANA V,DEMEDTS P’VanSANDE M,et al. A sim-ple and economical strategy for downstream processing of specific an-tibodies to human transferrin from egg yolk [ J ] . J Immunol Meth-ods, 1992,153( 1/2) :133 -140.
  • 4POLSON A, VON WECHMAR M B, Van REGENMORTEL M H.Isolation of viral IgY antibodes from yolks of immunized hens [ J].Immunol Commun,1980, 9(5) :475 -493.
  • 5HOHIKOSHI T,HIRAOKA J’SAITO M’et al. IgG antibody fromhen egg yolks:purification by ethanol fractionation[ J]. J Food Sci,1993,58(4):739 -742.
  • 6JENSENIUS J C,KOCH C. On the purification of IgG from egg yolk[J] . J Immunol MethodsT1993 ,164( 1) -.141 - 142.
  • 7DEIGNAN T,KELLY J, ALWAN A,et al. Comparative analysis ofmethods of purification of egg yolk immunoglobulin[ J]. Food AgricImmunol ,2000,12(1) :77 -85.
  • 8SRIRAM V,YGEESWARAN G, Improved recovery of immunoglobu-lin fraction from egg yolk of chicken immunized with Asialo GM1[J]. Russ J Immunol, 1999,4(2) : 132 - 140.
  • 9CONSTANTINOIU C C,MOLLOY J B,JORGENSEN W K,et al.Purification of immunoglobulins from chicken sera by thiophilic gelchromatography[ J]. Poultry Sci’2007 ,86(9) : 1910 - 1914.
  • 10McCANNEL A A,NAKAI S. Isolation of e yolk immunoglobulinsinto subpopulations using DEAE - ion exchange chromatography[J]. Can Inst Food Sci Technol,1989,23:42 -46.

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